Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice

Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementa...

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Autores principales:	Asadi,Ebrahim, Shabani,Ronak, Ghafari,Soraya, Golalipour,Mohammad Jafar
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Oocytes Morphine sulfate Vitamin E Apoptosis TUNEL assay Mice
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029
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spelling	oai:scielo:S0717-950220130002000292014-09-09Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated MiceAsadi,EbrahimShabani,RonakGhafari,SorayaGolalipour,Mohammad Jafar Oocytes Morphine sulfate Vitamin E Apoptosis TUNEL assay Mice Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10 Units) and 10 Unites of HCG. The next day the animals were sacrificed, oocytes were flushed from each fallopian tube. The collected oocytes were subjected to determine apoptosis by Tunnel assay with using Fluorescent Microscope. According to our results, the number of retrieved oocytes were 121, 132, 86 and 114 in control, experimental group I, II and III, respectively. Morphine Sulfate treatment increased apoptosis in oocytes to 17.44% whereas oocytes apoptosis was 4.13% in Controls. Supplementation with Vitamin E in Morphine Sulfate -treated mice reduced the oocytes apoptosis to 7.01%. This study showed that Morphine can increase apoptosis in oocytes and Vitamin E treatment significantly reduces oocytes apoptosis in the Morphine Sulfate -treated mice.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.2 20132013-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029en10.4067/S0717-95022013000200029
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Oocytes Morphine sulfate Vitamin E Apoptosis TUNEL assay Mice
spellingShingle	Oocytes Morphine sulfate Vitamin E Apoptosis TUNEL assay Mice Asadi,Ebrahim Shabani,Ronak Ghafari,Soraya Golalipour,Mohammad Jafar Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
description	Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10 Units) and 10 Unites of HCG. The next day the animals were sacrificed, oocytes were flushed from each fallopian tube. The collected oocytes were subjected to determine apoptosis by Tunnel assay with using Fluorescent Microscope. According to our results, the number of retrieved oocytes were 121, 132, 86 and 114 in control, experimental group I, II and III, respectively. Morphine Sulfate treatment increased apoptosis in oocytes to 17.44% whereas oocytes apoptosis was 4.13% in Controls. Supplementation with Vitamin E in Morphine Sulfate -treated mice reduced the oocytes apoptosis to 7.01%. This study showed that Morphine can increase apoptosis in oocytes and Vitamin E treatment significantly reduces oocytes apoptosis in the Morphine Sulfate -treated mice.
author	Asadi,Ebrahim Shabani,Ronak Ghafari,Soraya Golalipour,Mohammad Jafar
author_facet	Asadi,Ebrahim Shabani,Ronak Ghafari,Soraya Golalipour,Mohammad Jafar
author_sort	Asadi,Ebrahim
title	Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
title_short	Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
title_full	Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
title_fullStr	Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
title_full_unstemmed	Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
title_sort	preventing effect of vitamin e on oocytes apoptosis in morphine-treated mice
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029
work_keys_str_mv	AT asadiebrahim preventingeffectofvitamineonoocytesapoptosisinmorphinetreatedmice AT shabanironak preventingeffectofvitamineonoocytesapoptosisinmorphinetreatedmice AT ghafarisoraya preventingeffectofvitamineonoocytesapoptosisinmorphinetreatedmice AT golalipourmohammadjafar preventingeffectofvitamineonoocytesapoptosisinmorphinetreatedmice
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Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice

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