The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats

Several animal model studies have shown that Diabetes mellitus can affect on the activity of hippocampus astrocytes, but these studies reported controversial findings. This study was done to evaluate the preventive and treatment effect of Urtica dioica (U. dioica) on astrocytes density in the CA1 an...

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Autores principales:	Golalipour,Mohammad Jafar, Jahanshahi,Mehrdad, Ghafari,Soraya, Afshar,Mohammad
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Diabetes Astrocyte Urtica dioica Hippocampus CA1 CA3 Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200057
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spelling	oai:scielo:S0717-950220130002000572014-09-09The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic RatsGolalipour,Mohammad JafarJahanshahi,MehrdadGhafari,SorayaAfshar,Mohammad Diabetes Astrocyte Urtica dioica Hippocampus CA1 CA3 Rat Several animal model studies have shown that Diabetes mellitus can affect on the activity of hippocampus astrocytes, but these studies reported controversial findings. This study was done to evaluate the preventive and treatment effect of Urtica dioica (U. dioica) on astrocytes density in the CA1 and CA3 subfields of hippocampus of streptozotocin (STZ) induced diabetic rats. Twenty-eight male albino Wistar rats were randomly allocated equally into control, diabetic, U. dioica treatment and U. dioica preventive groups. Hyperglycemia was induced by STZ (80 mg/kg/BW). One week after injection of the streptozotocin, animals in treatment group were received hydroalcoholic extract of U. dioica (100 mg/kg/BW /day) for 4 weeks by intraperitoneally. In preventive group, diabetic rats were received 100 mg/kg/BW/ daily hydroalcoholic extract of U. dioica for 5 days before STZ injection. Then, animals were sacrificed and coronal sections were taken from the right dorsal hippocampus, stained with PTAH. The area densities of the astrocytes were measured. The number of astrocytes in CA1 of controls, diabetic treatment and preventive groups was 19.00±5.5, 17.14±6.4, 21±8.1 and 16.48±3.2, respectively. The densities of astrocytes in CA3 of controls, diabetic, treatment and preventive groups were 25.45±7.60, 21.54±7.5, 23.75±5.6 and 19.89±3.8, respectively. The density of astrocytes in diabetic rats reduced in comparison with controls (P<0.05). In CA1 and CA3, in spite of preventive administration, treatment of diabetic rats with U. dioica significantly increased the astrocytes. This study showed that treatment with U. dioica extract can help compensate for the CA1 and CA3 subfields of hippocampus astrocytes in diabetic rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.2 20132013-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200057en10.4067/S0717-95022013000200057
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diabetes Astrocyte Urtica dioica Hippocampus CA1 CA3 Rat
spellingShingle	Diabetes Astrocyte Urtica dioica Hippocampus CA1 CA3 Rat Golalipour,Mohammad Jafar Jahanshahi,Mehrdad Ghafari,Soraya Afshar,Mohammad The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
description	Several animal model studies have shown that Diabetes mellitus can affect on the activity of hippocampus astrocytes, but these studies reported controversial findings. This study was done to evaluate the preventive and treatment effect of Urtica dioica (U. dioica) on astrocytes density in the CA1 and CA3 subfields of hippocampus of streptozotocin (STZ) induced diabetic rats. Twenty-eight male albino Wistar rats were randomly allocated equally into control, diabetic, U. dioica treatment and U. dioica preventive groups. Hyperglycemia was induced by STZ (80 mg/kg/BW). One week after injection of the streptozotocin, animals in treatment group were received hydroalcoholic extract of U. dioica (100 mg/kg/BW /day) for 4 weeks by intraperitoneally. In preventive group, diabetic rats were received 100 mg/kg/BW/ daily hydroalcoholic extract of U. dioica for 5 days before STZ injection. Then, animals were sacrificed and coronal sections were taken from the right dorsal hippocampus, stained with PTAH. The area densities of the astrocytes were measured. The number of astrocytes in CA1 of controls, diabetic treatment and preventive groups was 19.00±5.5, 17.14±6.4, 21±8.1 and 16.48±3.2, respectively. The densities of astrocytes in CA3 of controls, diabetic, treatment and preventive groups were 25.45±7.60, 21.54±7.5, 23.75±5.6 and 19.89±3.8, respectively. The density of astrocytes in diabetic rats reduced in comparison with controls (P<0.05). In CA1 and CA3, in spite of preventive administration, treatment of diabetic rats with U. dioica significantly increased the astrocytes. This study showed that treatment with U. dioica extract can help compensate for the CA1 and CA3 subfields of hippocampus astrocytes in diabetic rats.
author	Golalipour,Mohammad Jafar Jahanshahi,Mehrdad Ghafari,Soraya Afshar,Mohammad
author_facet	Golalipour,Mohammad Jafar Jahanshahi,Mehrdad Ghafari,Soraya Afshar,Mohammad
author_sort	Golalipour,Mohammad Jafar
title	The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
title_short	The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
title_full	The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
title_fullStr	The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
title_full_unstemmed	The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats
title_sort	preventive and treatment effect of urtica dioica on astrocyte density in the ca1 and ca3 subfields of hippocampus in stz induced diabetic rats
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200057
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The Preventive and Treatment Effect of Urtica dioica on Astrocyte Density in the CA1 and CA3 Subfields of Hippocampus in STZ Induced Diabetic Rats

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