Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillo...

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Autores principales:	Catroxo,M. H. B, Martins,A. M. C. R. P. F, Petrella,S, Souza,F, Nastari,B.D.B
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Papillomavirus Cattle Transmission Electron Microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200068
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spelling	oai:scielo:S0717-950220130002000682014-09-09Ultrastructural Study of Bovine Papillomavirus During Outbreaks in BrazilCatroxo,M. H. BMartins,A. M. C. R. P. FPetrella,SSouza,FNastari,B.D.B Papillomavirus Cattle Transmission Electron Microscopy Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin lesion fragments. The ultrathin sections revealed the presence of groups of viral, intranuclear, rounded particles measuring 35 nm in diameter and tubular particles with a diameter of 35-39 nm. At immunoelectron microscopy technique, positivity obtained was marked by the presence of aggregates of viral particles formed by the antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction showed colloidal gold particles evenly distributed over the surface of the virus. These results showed the importance of the transmission electron microscopy techniques in the diagnosis of bovine papillomatosis that can be used in routine procedures to identify viral agent of this important disease.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.2 20132013-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200068en10.4067/S0717-95022013000200068
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Papillomavirus Cattle Transmission Electron Microscopy
spellingShingle	Papillomavirus Cattle Transmission Electron Microscopy Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Souza,F Nastari,B.D.B Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
description	Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin lesion fragments. The ultrathin sections revealed the presence of groups of viral, intranuclear, rounded particles measuring 35 nm in diameter and tubular particles with a diameter of 35-39 nm. At immunoelectron microscopy technique, positivity obtained was marked by the presence of aggregates of viral particles formed by the antigen-antibody interaction. In the immunocytochemistry technique, the antigen-antibody reaction showed colloidal gold particles evenly distributed over the surface of the virus. These results showed the importance of the transmission electron microscopy techniques in the diagnosis of bovine papillomatosis that can be used in routine procedures to identify viral agent of this important disease.
author	Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Souza,F Nastari,B.D.B
author_facet	Catroxo,M. H. B Martins,A. M. C. R. P. F Petrella,S Souza,F Nastari,B.D.B
author_sort	Catroxo,M. H. B
title	Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
title_short	Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
title_full	Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
title_fullStr	Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
title_full_unstemmed	Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil
title_sort	ultrastructural study of bovine papillomavirus during outbreaks in brazil
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200068
work_keys_str_mv	AT catroxomhb ultrastructuralstudyofbovinepapillomavirusduringoutbreaksinbrazil AT martinsamcrpf ultrastructuralstudyofbovinepapillomavirusduringoutbreaksinbrazil AT petrellas ultrastructuralstudyofbovinepapillomavirusduringoutbreaksinbrazil AT souzaf ultrastructuralstudyofbovinepapillomavirusduringoutbreaksinbrazil AT nastaribdb ultrastructuralstudyofbovinepapillomavirusduringoutbreaksinbrazil
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Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

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