Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture

Central nervous system of reptiles has the ability to grow and regenerate during adult life of the animal. Therefore, cells creating CNS of this animal class should compound substances or molecules enabling neuroregeneration. Cells directly involved in this process have not been clearly characterize...

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Autores principales: Grzesiak,Jakub, Marycz,Krzysztof, Szarek,Dariusz, Jarmundowicz,Wlodzimierz
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300008
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spelling oai:scielo:S0717-950220130003000082013-12-03Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in CultureGrzesiak,JakubMarycz,KrzysztofSzarek,DariuszJarmundowicz,Wlodzimierz Glial cells Neurogenesis Neuroregeneration Reptile Central nervous system of reptiles has the ability to grow and regenerate during adult life of the animal. Therefore, cells creating CNS of this animal class should compound substances or molecules enabling neuroregeneration. Cells directly involved in this process have not been clearly characterized, especially in cell culture environment. Morphology of reptilian glial adherent cells should be known better to find any differences from mammalian CNS cells. We isolated glial cells from olfactory bulb and cerebrum from gecko (Eublepharis macularius) and cultured separately. We have observed populations of cells with proliferative capacity in both types of cultures. Also, we have detected lipid molecules deposits within their cytoplasm, which localization was correlated with mitochondria position. This information can be helpful in searching new bioactive substances involved in regeneration of central nervous system.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300008en10.4067/S0717-95022013000300008
institution Scielo Chile
collection Scielo Chile
language English
topic Glial cells
Neurogenesis
Neuroregeneration
Reptile
spellingShingle Glial cells
Neurogenesis
Neuroregeneration
Reptile
Grzesiak,Jakub
Marycz,Krzysztof
Szarek,Dariusz
Jarmundowicz,Wlodzimierz
Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
description Central nervous system of reptiles has the ability to grow and regenerate during adult life of the animal. Therefore, cells creating CNS of this animal class should compound substances or molecules enabling neuroregeneration. Cells directly involved in this process have not been clearly characterized, especially in cell culture environment. Morphology of reptilian glial adherent cells should be known better to find any differences from mammalian CNS cells. We isolated glial cells from olfactory bulb and cerebrum from gecko (Eublepharis macularius) and cultured separately. We have observed populations of cells with proliferative capacity in both types of cultures. Also, we have detected lipid molecules deposits within their cytoplasm, which localization was correlated with mitochondria position. This information can be helpful in searching new bioactive substances involved in regeneration of central nervous system.
author Grzesiak,Jakub
Marycz,Krzysztof
Szarek,Dariusz
Jarmundowicz,Wlodzimierz
author_facet Grzesiak,Jakub
Marycz,Krzysztof
Szarek,Dariusz
Jarmundowicz,Wlodzimierz
author_sort Grzesiak,Jakub
title Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
title_short Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
title_full Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
title_fullStr Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
title_full_unstemmed Morphological Characterization of Gecko's (Eublepharis macularius) Glial Cells in Culture
title_sort morphological characterization of gecko's (eublepharis macularius) glial cells in culture
publisher Sociedad Chilena de Anatomía
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300008
work_keys_str_mv AT grzesiakjakub morphologicalcharacterizationofgeckoseublepharismaculariusglialcellsinculture
AT maryczkrzysztof morphologicalcharacterizationofgeckoseublepharismaculariusglialcellsinculture
AT szarekdariusz morphologicalcharacterizationofgeckoseublepharismaculariusglialcellsinculture
AT jarmundowiczwlodzimierz morphologicalcharacterizationofgeckoseublepharismaculariusglialcellsinculture
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