Ultrastructural Study of the Denervated Diaphragm in Rats

The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed aft...

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Autores principales:	Torrejais,M. M, Soares,J. C, Matheus,S. M. M, Mello,J. M, Francia-Farje,L. A. D, Jeronimo,L. C, Vicente,E. J. D
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Denervation Diaphragm Muscle fiber Ultrastructural Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300025
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spelling	oai:scielo:S0717-950220130003000252013-12-03Ultrastructural Study of the Denervated Diaphragm in RatsTorrejais,M. MSoares,J. CMatheus,S. M. MMello,J. MFrancia-Farje,L. A. DJeronimo,L. CVicente,E. J. D Denervation Diaphragm Muscle fiber Ultrastructural Rat The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300025en10.4067/S0717-95022013000300025
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Denervation Diaphragm Muscle fiber Ultrastructural Rat
spellingShingle	Denervation Diaphragm Muscle fiber Ultrastructural Rat Torrejais,M. M Soares,J. C Matheus,S. M. M Mello,J. M Francia-Farje,L. A. D Jeronimo,L. C Vicente,E. J. D Ultrastructural Study of the Denervated Diaphragm in Rats
description	The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.
author	Torrejais,M. M Soares,J. C Matheus,S. M. M Mello,J. M Francia-Farje,L. A. D Jeronimo,L. C Vicente,E. J. D
author_facet	Torrejais,M. M Soares,J. C Matheus,S. M. M Mello,J. M Francia-Farje,L. A. D Jeronimo,L. C Vicente,E. J. D
author_sort	Torrejais,M. M
title	Ultrastructural Study of the Denervated Diaphragm in Rats
title_short	Ultrastructural Study of the Denervated Diaphragm in Rats
title_full	Ultrastructural Study of the Denervated Diaphragm in Rats
title_fullStr	Ultrastructural Study of the Denervated Diaphragm in Rats
title_full_unstemmed	Ultrastructural Study of the Denervated Diaphragm in Rats
title_sort	ultrastructural study of the denervated diaphragm in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300025
work_keys_str_mv	AT torrejaismm ultrastructuralstudyofthedenervateddiaphragminrats AT soaresjc ultrastructuralstudyofthedenervateddiaphragminrats AT matheussmm ultrastructuralstudyofthedenervateddiaphragminrats AT mellojm ultrastructuralstudyofthedenervateddiaphragminrats AT franciafarjelad ultrastructuralstudyofthedenervateddiaphragminrats AT jeronimolc ultrastructuralstudyofthedenervateddiaphragminrats AT vicenteejd ultrastructuralstudyofthedenervateddiaphragminrats
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Ultrastructural Study of the Denervated Diaphragm in Rats

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