In vitro Development of Murine Embryos in presence of Campylobacter fetus

Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as cont...

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Autores principales:	Catena,M, Teruel,M, Morán,P, Chiapparrone,M, Echevarria,H, Soto,P
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Campylobacteriosis Blastocyst Morulae in vitro Mouse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400004
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spelling	oai:scielo:S0717-950220130004000042014-02-18In vitro Development of Murine Embryos in presence of Campylobacter fetusCatena,MTeruel,MMorán,PChiapparrone,MEchevarria,HSoto,P Campylobacteriosis Blastocyst Morulae in vitro Mouse Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as control group (CG). Treatment groups were constituted by the addition of Cfv 1 and 3, or Cff 2 and 5. Morulae were placed in Ham-F10 (CG); treatment groups were constituted by the addition of Cfv27, CFF (cell-free filtrate) and Brucella broth (BB). Blastocysts were cultured in MEM (CG); challenge group were constituted by the addition of Cfv 27. Differentiation, hatching, hatched, adhesion and expansion were evaluated. Results were analyzed by Chi2 test. In two-cell embryo, the differentiation rate was not modified when the study strains were added (p > 0.05). The differentiation rate at 24 h for embryos at the morula stage was lower for BB, Cfv, and CFF, compared with CG (p < 0.05). After 48 h culture, no differences were observed in blastocyst formation for Cfv and BB, compared to CG (p > 0.05). However, the differentiation rate for the CFF group was lower than for CG (p < 0.05). At 48 and 72 h, the hatching rate was higher in CFF and Cfv groups than in CG (p < 0.05). Differences were not detected in blastocyst cultures. In conclusion, under these experimental conditions, Cf was not detrimental to the development of murine embryos. Efforts will be intensified to establish in vitro infection models that reproduce their pathogenicity.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400004en10.4067/S0717-95022013000400004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Campylobacteriosis Blastocyst Morulae in vitro Mouse
spellingShingle	Campylobacteriosis Blastocyst Morulae in vitro Mouse Catena,M Teruel,M Morán,P Chiapparrone,M Echevarria,H Soto,P In vitro Development of Murine Embryos in presence of Campylobacter fetus
description	Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as control group (CG). Treatment groups were constituted by the addition of Cfv 1 and 3, or Cff 2 and 5. Morulae were placed in Ham-F10 (CG); treatment groups were constituted by the addition of Cfv27, CFF (cell-free filtrate) and Brucella broth (BB). Blastocysts were cultured in MEM (CG); challenge group were constituted by the addition of Cfv 27. Differentiation, hatching, hatched, adhesion and expansion were evaluated. Results were analyzed by Chi2 test. In two-cell embryo, the differentiation rate was not modified when the study strains were added (p > 0.05). The differentiation rate at 24 h for embryos at the morula stage was lower for BB, Cfv, and CFF, compared with CG (p < 0.05). After 48 h culture, no differences were observed in blastocyst formation for Cfv and BB, compared to CG (p > 0.05). However, the differentiation rate for the CFF group was lower than for CG (p < 0.05). At 48 and 72 h, the hatching rate was higher in CFF and Cfv groups than in CG (p < 0.05). Differences were not detected in blastocyst cultures. In conclusion, under these experimental conditions, Cf was not detrimental to the development of murine embryos. Efforts will be intensified to establish in vitro infection models that reproduce their pathogenicity.
author	Catena,M Teruel,M Morán,P Chiapparrone,M Echevarria,H Soto,P
author_facet	Catena,M Teruel,M Morán,P Chiapparrone,M Echevarria,H Soto,P
author_sort	Catena,M
title	In vitro Development of Murine Embryos in presence of Campylobacter fetus
title_short	In vitro Development of Murine Embryos in presence of Campylobacter fetus
title_full	In vitro Development of Murine Embryos in presence of Campylobacter fetus
title_fullStr	In vitro Development of Murine Embryos in presence of Campylobacter fetus
title_full_unstemmed	In vitro Development of Murine Embryos in presence of Campylobacter fetus
title_sort	in vitro development of murine embryos in presence of campylobacter fetus
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400004
work_keys_str_mv	AT catenam invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus AT teruelm invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus AT moranp invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus AT chiapparronem invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus AT echevarriah invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus AT sotop invitrodevelopmentofmurineembryosinpresenceofcampylobacterfetus
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In vitro Development of Murine Embryos in presence of Campylobacter fetus

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