Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus

Cholinergic system in CNS is involved in learning and memory. Scopolamine as muscarinic acetylcholine receptor antagonist is used for creation of memory impairment. The purpose of this study is evaluation of scopolamine-based amnesia on memory retention and the effect of this phenomenon on the numbe...

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Autores principales:	Jahanshahi,M, Nickmahzar,E. G, Seif-hoseini,S, Babakordi,F, Moharreri,A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	M1 receptor Scopolamine Avoidance memory Hippocampus Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400014
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spelling	oai:scielo:S0717-950220130004000142014-02-18Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' HippocampusJahanshahi,MNickmahzar,E. GSeif-hoseini,SBabakordi,FMoharreri,A M1 receptor Scopolamine Avoidance memory Hippocampus Rat Cholinergic system in CNS is involved in learning and memory. Scopolamine as muscarinic acetylcholine receptor antagonist is used for creation of memory impairment. The purpose of this study is evaluation of scopolamine-based amnesia on memory retention and the effect of this phenomenon on the number of neurons contains M1-receptors in the male Wistar rats hippocampal regions. Thirty-five male Wistar rats (200±20 g) were distributed randomly into five groups. Control group (intact samples) and 3 experimental groups with sham group (saline) were tested by the method of passive avoidance (shuttle box) in doses of 0.2, 0.5 and 1 mg/kg (intraperitoneally) as a single dose. After one week, memory test was taken from the rats. Finally, brains dissected from sacrificed rats, and then processed tissues were stained with antibody against M1 receptors (Immunohistochemistry technique) followed by counting of hippocampal CA1, CA3 and DG regions. Our results showed significant decrease in neurons contains M1-receptors in all area of hippocampus. We found that the less number of M1-neurons showed in 1 mg/kg dose of scopolamine. We concluded that scopolamine as muscarinic acetylcholine receptor antagonist can reduce dose-dependently the density of M1-neurons in all areas of hippocampus.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400014en10.4067/S0717-95022013000400014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	M1 receptor Scopolamine Avoidance memory Hippocampus Rat
spellingShingle	M1 receptor Scopolamine Avoidance memory Hippocampus Rat Jahanshahi,M Nickmahzar,E. G Seif-hoseini,S Babakordi,F Moharreri,A Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
description	Cholinergic system in CNS is involved in learning and memory. Scopolamine as muscarinic acetylcholine receptor antagonist is used for creation of memory impairment. The purpose of this study is evaluation of scopolamine-based amnesia on memory retention and the effect of this phenomenon on the number of neurons contains M1-receptors in the male Wistar rats hippocampal regions. Thirty-five male Wistar rats (200±20 g) were distributed randomly into five groups. Control group (intact samples) and 3 experimental groups with sham group (saline) were tested by the method of passive avoidance (shuttle box) in doses of 0.2, 0.5 and 1 mg/kg (intraperitoneally) as a single dose. After one week, memory test was taken from the rats. Finally, brains dissected from sacrificed rats, and then processed tissues were stained with antibody against M1 receptors (Immunohistochemistry technique) followed by counting of hippocampal CA1, CA3 and DG regions. Our results showed significant decrease in neurons contains M1-receptors in all area of hippocampus. We found that the less number of M1-neurons showed in 1 mg/kg dose of scopolamine. We concluded that scopolamine as muscarinic acetylcholine receptor antagonist can reduce dose-dependently the density of M1-neurons in all areas of hippocampus.
author	Jahanshahi,M Nickmahzar,E. G Seif-hoseini,S Babakordi,F Moharreri,A
author_facet	Jahanshahi,M Nickmahzar,E. G Seif-hoseini,S Babakordi,F Moharreri,A
author_sort	Jahanshahi,M
title	Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
title_short	Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
title_full	Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
title_fullStr	Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
title_full_unstemmed	Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus
title_sort	scopolamine reduces the density of m1 muscarinic neurons in rats' hippocampus
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400014
work_keys_str_mv	AT jahanshahim scopolaminereducesthedensityofm1muscarinicneuronsinratshippocampus AT nickmahzareg scopolaminereducesthedensityofm1muscarinicneuronsinratshippocampus AT seifhoseinis scopolaminereducesthedensityofm1muscarinicneuronsinratshippocampus AT babakordif scopolaminereducesthedensityofm1muscarinicneuronsinratshippocampus AT moharreria scopolaminereducesthedensityofm1muscarinicneuronsinratshippocampus
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Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus

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