Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro

Centella asiatica or "pegaga" is well known for its ability in promoting wound healing. This study focused on the effect of Centella asiatica on the proliferation of human respiratory epithelial (RE) cells. RE cells were cultured using co-culture techniques until first passage (P1). Viabil...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mutua,P. M, Gicheru,M. M, Makanya,A. N, Kiama,S. G
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2013
Materias:	Centella asiatica Respiratory epithelial cells Anti-proliferative
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400028
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022013000400028
record_format	dspace
spelling	oai:scielo:S0717-950220130004000282014-02-18Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitroMutua,P. MGicheru,M. MMakanya,A. NKiama,S. G Centella asiatica Respiratory epithelial cells Anti-proliferative Centella asiatica or "pegaga" is well known for its ability in promoting wound healing. This study focused on the effect of Centella asiatica on the proliferation of human respiratory epithelial (RE) cells. RE cells were cultured using co-culture techniques until first passage (P1). Viability cell test by tryphan blue dye exclusion assay showed that there was high percentage of cell viability at both P0 (74%) and P1 (91.61%). Triplicate MTT assays were carried out with different concentrations of C. asiatica from 15.6 ppm, 31.3 ppm, 62.5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm, until 2000 ppm. The higher the concentration of C. asiatica, the more inhibitory effect was seen. C. asiatica aqueous extract at concentration 1000 ppm and 2000 ppm demonstrated a significant (p<0.05) inhibitory effect on human RE cells proliferation on day 4 and day 7 after treatment. This provides potential use of C. asiatica extract for the treatment of conditions with respiratory epithelial cells overgrowth.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.31 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400028en10.4067/S0717-95022013000400028
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Centella asiatica Respiratory epithelial cells Anti-proliferative
spellingShingle	Centella asiatica Respiratory epithelial cells Anti-proliferative Mutua,P. M Gicheru,M. M Makanya,A. N Kiama,S. G Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
description	Centella asiatica or "pegaga" is well known for its ability in promoting wound healing. This study focused on the effect of Centella asiatica on the proliferation of human respiratory epithelial (RE) cells. RE cells were cultured using co-culture techniques until first passage (P1). Viability cell test by tryphan blue dye exclusion assay showed that there was high percentage of cell viability at both P0 (74%) and P1 (91.61%). Triplicate MTT assays were carried out with different concentrations of C. asiatica from 15.6 ppm, 31.3 ppm, 62.5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm, until 2000 ppm. The higher the concentration of C. asiatica, the more inhibitory effect was seen. C. asiatica aqueous extract at concentration 1000 ppm and 2000 ppm demonstrated a significant (p<0.05) inhibitory effect on human RE cells proliferation on day 4 and day 7 after treatment. This provides potential use of C. asiatica extract for the treatment of conditions with respiratory epithelial cells overgrowth.
author	Mutua,P. M Gicheru,M. M Makanya,A. N Kiama,S. G
author_facet	Mutua,P. M Gicheru,M. M Makanya,A. N Kiama,S. G
author_sort	Mutua,P. M
title	Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
title_short	Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
title_full	Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
title_fullStr	Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
title_full_unstemmed	Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro
title_sort	anti-proliferative activities of centella asiatica extracts on human respiratory epithelial cells in vitro
publisher	Sociedad Chilena de Anatomía
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400028
work_keys_str_mv	AT mutuapm antiproliferativeactivitiesofcentellaasiaticaextractsonhumanrespiratoryepithelialcellsinvitro AT gicherumm antiproliferativeactivitiesofcentellaasiaticaextractsonhumanrespiratoryepithelialcellsinvitro AT makanyaan antiproliferativeactivitiesofcentellaasiaticaextractsonhumanrespiratoryepithelialcellsinvitro AT kiamasg antiproliferativeactivitiesofcentellaasiaticaextractsonhumanrespiratoryepithelialcellsinvitro
_version_	1718444847135719424

Anti-Proliferative Activities of Centella Asiatica Extracts on Human Respiratory Epithelial Cells in vitro

Ejemplares similares