Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line

Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Montiel-Eulefi,E, Jara,F, Toro,C, Garcés,M, Leal,P
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Copper loaded nanoparticles Poly-lactic acid Adenocarcinoma MKN45cells Cytotoxicity Cell cycle
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022014000100011
record_format	dspace
spelling	oai:scielo:S0717-950220140001000112015-11-13Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell LineMontiel-Eulefi,EJara,FToro,CGarcés,MLeal,P Copper loaded nanoparticles Poly-lactic acid Adenocarcinoma MKN45cells Cytotoxicity Cell cycle Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate the cytotoxic, apoptotic and cell cycle properties of PLA and CuSO4-loaded PLA biodegradable nanoparticles on MKN-45 gastric adenocarcinoma cell line. PLA nanoparticles for the delivery of the anticancer active principle CuSO4 were obtained using the double emulsion method. PLA and CuSO4 loaded PLA nanoparticles were morphologically characterized and their size determined using transmission electron microscopy (TEM). The cytotoxicity of this drug delivery system was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; apoptosis was evaluated using YO-PRO-1/Propidium Iodide and cell cycle analysis throughout flow cytometry. CuSO4-loaded PLA nanoparticles were effective inhibitors of MKN45 cancer cell growth. They increased cytotoxicity and apoptosis, and induced G1/Go cell cycle arrest;whereas the anticancer activity was increased using a 96 h treatment of a minimal (1mM) concentration of CuSO4 loaded in 40 µM PLA nanoparticles. The treatment with 40 µM lactic acid and PLA (40 µM) did not increase the rate of cell survival assays related to the control, which indicate that PLA use as a polymer carrier not induce proliferation of MKN-45 cancer cells. Our research presents novel data about the effect of PLA nanoparticles and CuSO4 on gastric cancer cell line MKN45.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.1 20142014-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011en10.4067/S0717-95022014000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Copper loaded nanoparticles Poly-lactic acid Adenocarcinoma MKN45cells Cytotoxicity Cell cycle
spellingShingle	Copper loaded nanoparticles Poly-lactic acid Adenocarcinoma MKN45cells Cytotoxicity Cell cycle Montiel-Eulefi,E Jara,F Toro,C Garcés,M Leal,P Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
description	Poly (L-lactic acid) (PLA) nanoparticles have the approval of the main institutions for drugs administration and therapeutics. However, the use of lactic acid polymer is controversial because lactic acid has been proposed as an energy source for cancer cells. The aim of this study was to evaluate the cytotoxic, apoptotic and cell cycle properties of PLA and CuSO4-loaded PLA biodegradable nanoparticles on MKN-45 gastric adenocarcinoma cell line. PLA nanoparticles for the delivery of the anticancer active principle CuSO4 were obtained using the double emulsion method. PLA and CuSO4 loaded PLA nanoparticles were morphologically characterized and their size determined using transmission electron microscopy (TEM). The cytotoxicity of this drug delivery system was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; apoptosis was evaluated using YO-PRO-1/Propidium Iodide and cell cycle analysis throughout flow cytometry. CuSO4-loaded PLA nanoparticles were effective inhibitors of MKN45 cancer cell growth. They increased cytotoxicity and apoptosis, and induced G1/Go cell cycle arrest;whereas the anticancer activity was increased using a 96 h treatment of a minimal (1mM) concentration of CuSO4 loaded in 40 µM PLA nanoparticles. The treatment with 40 µM lactic acid and PLA (40 µM) did not increase the rate of cell survival assays related to the control, which indicate that PLA use as a polymer carrier not induce proliferation of MKN-45 cancer cells. Our research presents novel data about the effect of PLA nanoparticles and CuSO4 on gastric cancer cell line MKN45.
author	Montiel-Eulefi,E Jara,F Toro,C Garcés,M Leal,P
author_facet	Montiel-Eulefi,E Jara,F Toro,C Garcés,M Leal,P
author_sort	Montiel-Eulefi,E
title	Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
title_short	Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
title_full	Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
title_fullStr	Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
title_full_unstemmed	Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line
title_sort	cytotoxic effect of double emulsion (w/o/w) cuso4 loaded pla nanoparticles on mkn-45 gastric adenocarcinoma cell line
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100011
work_keys_str_mv	AT montieleulefie cytotoxiceffectofdoubleemulsionwowcuso4loadedplananoparticlesonmkn45gastricadenocarcinomacellline AT jaraf cytotoxiceffectofdoubleemulsionwowcuso4loadedplananoparticlesonmkn45gastricadenocarcinomacellline AT toroc cytotoxiceffectofdoubleemulsionwowcuso4loadedplananoparticlesonmkn45gastricadenocarcinomacellline AT garcesm cytotoxiceffectofdoubleemulsionwowcuso4loadedplananoparticlesonmkn45gastricadenocarcinomacellline AT lealp cytotoxiceffectofdoubleemulsionwowcuso4loadedplananoparticlesonmkn45gastricadenocarcinomacellline
_version_	1718444855095459840

Cytotoxic Effect of Double Emulsion (W/O/W) CuSO4 Loaded PLA Nanoparticles on MKN-45 Gastric Adenocarcinoma Cell Line

Ejemplares similares