Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life

Previous study has shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal model. This study was conducted to determine the effect of gestational diabetes on rat cerebellum in early postnatal life. In this experimental study, 10 dams randomly allocated into contro...

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Autores principales:	Razi,Elahe Mirarab, Ghafari,Soraya, Hojati,Vida, Golalipour,Mohammad Jafar
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Gestational diabetes Cerebellum Purkinje cell Granular cell Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200006
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spelling	oai:scielo:S0717-950220140002000062015-11-16Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal LifeRazi,Elahe MirarabGhafari,SorayaHojati,VidaGolalipour,Mohammad Jafar Gestational diabetes Cerebellum Purkinje cell Granular cell Rat Previous study has shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal model. This study was conducted to determine the effect of gestational diabetes on rat cerebellum in early postnatal life. In this experimental study, 10 dams randomly allocated into control and diabetic groups on day 1 of gestation. Five dams in diabetic group were administered 40 mg/kg/BW (intraperitoneally) of streptozotocin and control animals received normal saline. Six offspring of each gestational diabetes mellitus and controls were randomly selected at day 7 of postnatal life. Offspring were sacrificed and coronal sections were taken from the cerebellum and stained with cresyl violet. The number of Purkinje and granular cells and thickness of layers of cerebellum were evaluated by quantitative computer-assisted morphometric method. The Purkinje cells density at apex and depth of cerebellar lobules in the experimental group (14.40±0.7, 14.86±0.6) significantly reduced in comparison with the control group (16.72±0.3, 17.85±0.7) (P<0.05). The granular cell density at apex and depth of cerebellar lobules in the experimental group (23.94±0.6, 22.81±0.5) significantly reduced in comparison with the control group (29.20±0.8, 28.1±0.8) (P<0.05). The thickness of the Purkinje and internal granular and molecular layers at apex and depth of cerebellar cortex significantly reduced in diabetics group compared to controls (P<0.05). This study revealed that gestational diabetes induces loss of number and size of the Purkinje cells and the granular cells and reduction of thickness of the Purkinje and internal granular layer of the cerebellar cortex in neonatal mice.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.2 20142014-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200006en10.4067/S0717-95022014000200006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gestational diabetes Cerebellum Purkinje cell Granular cell Rat
spellingShingle	Gestational diabetes Cerebellum Purkinje cell Granular cell Rat Razi,Elahe Mirarab Ghafari,Soraya Hojati,Vida Golalipour,Mohammad Jafar Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
description	Previous study has shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal model. This study was conducted to determine the effect of gestational diabetes on rat cerebellum in early postnatal life. In this experimental study, 10 dams randomly allocated into control and diabetic groups on day 1 of gestation. Five dams in diabetic group were administered 40 mg/kg/BW (intraperitoneally) of streptozotocin and control animals received normal saline. Six offspring of each gestational diabetes mellitus and controls were randomly selected at day 7 of postnatal life. Offspring were sacrificed and coronal sections were taken from the cerebellum and stained with cresyl violet. The number of Purkinje and granular cells and thickness of layers of cerebellum were evaluated by quantitative computer-assisted morphometric method. The Purkinje cells density at apex and depth of cerebellar lobules in the experimental group (14.40±0.7, 14.86±0.6) significantly reduced in comparison with the control group (16.72±0.3, 17.85±0.7) (P<0.05). The granular cell density at apex and depth of cerebellar lobules in the experimental group (23.94±0.6, 22.81±0.5) significantly reduced in comparison with the control group (29.20±0.8, 28.1±0.8) (P<0.05). The thickness of the Purkinje and internal granular and molecular layers at apex and depth of cerebellar cortex significantly reduced in diabetics group compared to controls (P<0.05). This study revealed that gestational diabetes induces loss of number and size of the Purkinje cells and the granular cells and reduction of thickness of the Purkinje and internal granular layer of the cerebellar cortex in neonatal mice.
author	Razi,Elahe Mirarab Ghafari,Soraya Hojati,Vida Golalipour,Mohammad Jafar
author_facet	Razi,Elahe Mirarab Ghafari,Soraya Hojati,Vida Golalipour,Mohammad Jafar
author_sort	Razi,Elahe Mirarab
title	Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
title_short	Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
title_full	Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
title_fullStr	Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
title_full_unstemmed	Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life
title_sort	effect of gestational diabetes on neuronal cells in rat cerebellum in early postnatal life
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200006
work_keys_str_mv	AT razielahemirarab effectofgestationaldiabetesonneuronalcellsinratcerebelluminearlypostnatallife AT ghafarisoraya effectofgestationaldiabetesonneuronalcellsinratcerebelluminearlypostnatallife AT hojativida effectofgestationaldiabetesonneuronalcellsinratcerebelluminearlypostnatallife AT golalipourmohammadjafar effectofgestationaldiabetesonneuronalcellsinratcerebelluminearlypostnatallife
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Effect of Gestational Diabetes on Neuronal Cells in Rat Cerebellum in Early Postnatal Life

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