Morphological Alterations in Mouse Placenta Induced by Diazepam

Diazepam (DZ) is a benzodiazepine that belongs to the group of minor tranquilizers with myo-relaxing and anticonvulsant properties. DZ and its metabolites cross the placental barrier in human, monkey, hamster, and mouse, and accumulate in the placenta. Our aim was to investigate, through histologica...

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Autores principales:	Márquez-Orozco,María Cristina, Gasca-Ramírez,María Verónica, de la Fuente-Juárez,Graciela, Márquez-Orozco,Amalia
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Benzodiazepine Diazepam Mouse Placenta Histology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200007
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spelling	oai:scielo:S0717-950220140002000072015-11-16Morphological Alterations in Mouse Placenta Induced by DiazepamMárquez-Orozco,María CristinaGasca-Ramírez,María Verónicade la Fuente-Juárez,GracielaMárquez-Orozco,Amalia Benzodiazepine Diazepam Mouse Placenta Histology Diazepam (DZ) is a benzodiazepine that belongs to the group of minor tranquilizers with myo-relaxing and anticonvulsant properties. DZ and its metabolites cross the placental barrier in human, monkey, hamster, and mouse, and accumulate in the placenta. Our aim was to investigate, through histological techniques, and semifine sections if DZ induces morphological changes in the placenta. Twenty female mice of the ICR strain were distributed randomly in two groups. One group (DZ) was treated from days 6 to 17 of gestation with a single daily subcutaneous (sc) dose of DZ of 2.7 mg/kg/ (bw); the second, control group (C) was treated with saline solution. All females (10 DZ and 10 C) were killed by decapitation. Placentas were extracted and fixed in phosphates-buffered 10% formaldehyde, pH 7.3, dehydrated, and embedded in paraffin to obtain 3 µm thick sections or fixed in 2.5% glutaraldehyde, post-fixed in 1% OsO4, embedded in epoxy resin. Histological sections were stained with hematoxylin-eosin or Weigert´s iron hematoxylin. Semifine sections were stained with toluidine blue. All sections were observed under comparative light microscopy. The DZ-group showed thinned placental barrier with multiple vacuoles. Nuclei of trophoblast cells (TCs) and trophoblast giant cells (TGCs) presented heterochromatin in coarse granules, atypically distributed in the karyolymph and conspicuous nucleoli. The cytoplasm of the TGCs was vacuolated and chromatin had a similar appearance to that observed in TCs. The total area of the placental barrier was measured in µm2/µm2; the area in the DZ group was reduced as compared with the C group (P<0.001). Alterations of TGCs could be due to an interaction of DZ with peripheral type benzodiazepine receptors involved in progesterone biosynthesis. Administration of DZ in mice alters the placental barrier and TGCs which could affect their physiology and causes teratogenic effects on the ovary and testis involved in steroid hormones biosynthesis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.2 20142014-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200007en10.4067/S0717-95022014000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Benzodiazepine Diazepam Mouse Placenta Histology
spellingShingle	Benzodiazepine Diazepam Mouse Placenta Histology Márquez-Orozco,María Cristina Gasca-Ramírez,María Verónica de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia Morphological Alterations in Mouse Placenta Induced by Diazepam
description	Diazepam (DZ) is a benzodiazepine that belongs to the group of minor tranquilizers with myo-relaxing and anticonvulsant properties. DZ and its metabolites cross the placental barrier in human, monkey, hamster, and mouse, and accumulate in the placenta. Our aim was to investigate, through histological techniques, and semifine sections if DZ induces morphological changes in the placenta. Twenty female mice of the ICR strain were distributed randomly in two groups. One group (DZ) was treated from days 6 to 17 of gestation with a single daily subcutaneous (sc) dose of DZ of 2.7 mg/kg/ (bw); the second, control group (C) was treated with saline solution. All females (10 DZ and 10 C) were killed by decapitation. Placentas were extracted and fixed in phosphates-buffered 10% formaldehyde, pH 7.3, dehydrated, and embedded in paraffin to obtain 3 µm thick sections or fixed in 2.5% glutaraldehyde, post-fixed in 1% OsO4, embedded in epoxy resin. Histological sections were stained with hematoxylin-eosin or Weigert´s iron hematoxylin. Semifine sections were stained with toluidine blue. All sections were observed under comparative light microscopy. The DZ-group showed thinned placental barrier with multiple vacuoles. Nuclei of trophoblast cells (TCs) and trophoblast giant cells (TGCs) presented heterochromatin in coarse granules, atypically distributed in the karyolymph and conspicuous nucleoli. The cytoplasm of the TGCs was vacuolated and chromatin had a similar appearance to that observed in TCs. The total area of the placental barrier was measured in µm2/µm2; the area in the DZ group was reduced as compared with the C group (P<0.001). Alterations of TGCs could be due to an interaction of DZ with peripheral type benzodiazepine receptors involved in progesterone biosynthesis. Administration of DZ in mice alters the placental barrier and TGCs which could affect their physiology and causes teratogenic effects on the ovary and testis involved in steroid hormones biosynthesis.
author	Márquez-Orozco,María Cristina Gasca-Ramírez,María Verónica de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia
author_facet	Márquez-Orozco,María Cristina Gasca-Ramírez,María Verónica de la Fuente-Juárez,Graciela Márquez-Orozco,Amalia
author_sort	Márquez-Orozco,María Cristina
title	Morphological Alterations in Mouse Placenta Induced by Diazepam
title_short	Morphological Alterations in Mouse Placenta Induced by Diazepam
title_full	Morphological Alterations in Mouse Placenta Induced by Diazepam
title_fullStr	Morphological Alterations in Mouse Placenta Induced by Diazepam
title_full_unstemmed	Morphological Alterations in Mouse Placenta Induced by Diazepam
title_sort	morphological alterations in mouse placenta induced by diazepam
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200007
work_keys_str_mv	AT marquezorozcomariacristina morphologicalalterationsinmouseplacentainducedbydiazepam AT gascaramirezmariaveronica morphologicalalterationsinmouseplacentainducedbydiazepam AT delafuentejuarezgraciela morphologicalalterationsinmouseplacentainducedbydiazepam AT marquezorozcoamalia morphologicalalterationsinmouseplacentainducedbydiazepam
_version_	1718444867232727040

Morphological Alterations in Mouse Placenta Induced by Diazepam

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