Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions

The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of heal...

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Autores principales:	Sandoval,Catherine, Nuñez,Marcelo, Roa,Ignacio
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Barr body Fibroblast Dental pulp Sex identification Burial conditions
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200026
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spelling	oai:scielo:S0717-950220140002000262015-11-16Dental Pulp Fibroblast and Sex Determination in Controlled Burial ConditionsSandoval,CatherineNuñez,MarceloRoa,Ignacio Barr body Fibroblast Dental pulp Sex identification Burial conditions The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of healthy teeth, under different conditions of burial (in vitro) with variations in pH, humidity and salinity in terms of general accuracy and sensitivity for men and women. Analyzed sample considered 47 dental pulps, taken from teeth under burial conditions during a period of a month. From dental pulps samples, 265 histological cuts valid for this study, were obtained, which were observed with an optical microscope under conventional H/E staining. Results showed a 98.9% of well-diagnosed cases, which correspond to the overall accuracy of the method. Sensitivity for men was 97.5% and 100% for women, over the analyzed sample. In low humidity conditions, 3 samples of badly diagnosed cases in men were observed, with a group accuracy of a 90%, with a sensitivity of 25% for men and 100% for women. The present study establishes that based on these results, the performance of Barr Body Test in fibroblasts, proposed for healthy pulp teeth, is not affected by burial conditions in terms of pH (acid-alkaline), salinity (high-low) and high humidity.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.2 20142014-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200026en10.4067/S0717-95022014000200026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Barr body Fibroblast Dental pulp Sex identification Burial conditions
spellingShingle	Barr body Fibroblast Dental pulp Sex identification Burial conditions Sandoval,Catherine Nuñez,Marcelo Roa,Ignacio Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
description	The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of healthy teeth, under different conditions of burial (in vitro) with variations in pH, humidity and salinity in terms of general accuracy and sensitivity for men and women. Analyzed sample considered 47 dental pulps, taken from teeth under burial conditions during a period of a month. From dental pulps samples, 265 histological cuts valid for this study, were obtained, which were observed with an optical microscope under conventional H/E staining. Results showed a 98.9% of well-diagnosed cases, which correspond to the overall accuracy of the method. Sensitivity for men was 97.5% and 100% for women, over the analyzed sample. In low humidity conditions, 3 samples of badly diagnosed cases in men were observed, with a group accuracy of a 90%, with a sensitivity of 25% for men and 100% for women. The present study establishes that based on these results, the performance of Barr Body Test in fibroblasts, proposed for healthy pulp teeth, is not affected by burial conditions in terms of pH (acid-alkaline), salinity (high-low) and high humidity.
author	Sandoval,Catherine Nuñez,Marcelo Roa,Ignacio
author_facet	Sandoval,Catherine Nuñez,Marcelo Roa,Ignacio
author_sort	Sandoval,Catherine
title	Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
title_short	Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
title_full	Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
title_fullStr	Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
title_full_unstemmed	Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions
title_sort	dental pulp fibroblast and sex determination in controlled burial conditions
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200026
work_keys_str_mv	AT sandovalcatherine dentalpulpfibroblastandsexdeterminationincontrolledburialconditions AT nunezmarcelo dentalpulpfibroblastandsexdeterminationincontrolledburialconditions AT roaignacio dentalpulpfibroblastandsexdeterminationincontrolledburialconditions
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Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions

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