Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage

Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin an...

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Autores principales:	Hatakeyama,Yuji, Hatakeyama,Junko, Oka,Kyoko, Tsuruga,Eichi, Inai,Tetsuichiro, Anan,Hisashi, Sawa,Yoshihiko
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Amelogenin Lysosome-associated membrane proteins (LAMPs) Chondrogenesis Cartilage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200040
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spelling	oai:scielo:S0717-950220140002000402015-11-16Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in CartilageHatakeyama,YujiHatakeyama,JunkoOka,KyokoTsuruga,EichiInai,TetsuichiroAnan,HisashiSawa,Yoshihiko Amelogenin Lysosome-associated membrane proteins (LAMPs) Chondrogenesis Cartilage Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin and it has been suggested they act as signaling receptors of amelogenin. The purpose of this study is to clarify the localization of amelogenin and LAMPs in growth plate cartilage and cartilaginous nodules in micromass culture. Mouse knee joints including tibia growth plate at 4 weeks old and micromass cultures of limb bud mesenchymal cells after 2 weeks were fixed in paraformaldehyde, routinely processed, sections were cut and immunostained with amelogenin, collagen type II and type X, LAMP-1 and -3. The positive immunoreaction of amelogenin was observed both in proliferation and hypertrophic zone cartilage of growth plate after enzymatic pretreatment in immunostaining. Furthermore, cartilaginous nodules in micromass culture were immunopositive to amelogenin. The chondrocytes in the proliferation zone of the growth plate were immunopositive to LAMP-1 but weakly stained in the chondrocytes of hypertrophic zone. These observations indicate that amelogenin may be present in cartilage matrix produced in vivo and in vitro and amelogenin may involve cartilage formation through the LAMP-1 signaling pathway.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.2 20142014-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200040en10.4067/S0717-95022014000200040
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Amelogenin Lysosome-associated membrane proteins (LAMPs) Chondrogenesis Cartilage
spellingShingle	Amelogenin Lysosome-associated membrane proteins (LAMPs) Chondrogenesis Cartilage Hatakeyama,Yuji Hatakeyama,Junko Oka,Kyoko Tsuruga,Eichi Inai,Tetsuichiro Anan,Hisashi Sawa,Yoshihiko Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
description	Amelogenin is one of the enamel matrix proteins secreted by ameloblasts during enamel formation in tooth development. Recent studies showed that the amelogenin is expressed in chondrocyte. Lysosome-associated membrane proteins (LAMPs) have been identified as binding partner proteins to amelogenin and it has been suggested they act as signaling receptors of amelogenin. The purpose of this study is to clarify the localization of amelogenin and LAMPs in growth plate cartilage and cartilaginous nodules in micromass culture. Mouse knee joints including tibia growth plate at 4 weeks old and micromass cultures of limb bud mesenchymal cells after 2 weeks were fixed in paraformaldehyde, routinely processed, sections were cut and immunostained with amelogenin, collagen type II and type X, LAMP-1 and -3. The positive immunoreaction of amelogenin was observed both in proliferation and hypertrophic zone cartilage of growth plate after enzymatic pretreatment in immunostaining. Furthermore, cartilaginous nodules in micromass culture were immunopositive to amelogenin. The chondrocytes in the proliferation zone of the growth plate were immunopositive to LAMP-1 but weakly stained in the chondrocytes of hypertrophic zone. These observations indicate that amelogenin may be present in cartilage matrix produced in vivo and in vitro and amelogenin may involve cartilage formation through the LAMP-1 signaling pathway.
author	Hatakeyama,Yuji Hatakeyama,Junko Oka,Kyoko Tsuruga,Eichi Inai,Tetsuichiro Anan,Hisashi Sawa,Yoshihiko
author_facet	Hatakeyama,Yuji Hatakeyama,Junko Oka,Kyoko Tsuruga,Eichi Inai,Tetsuichiro Anan,Hisashi Sawa,Yoshihiko
author_sort	Hatakeyama,Yuji
title	Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
title_short	Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
title_full	Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
title_fullStr	Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
title_full_unstemmed	Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage
title_sort	immunohistochemical study of amelogenin and lysosome-associate membrane proteins (lamps) in cartilage
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200040
work_keys_str_mv	AT hatakeyamayuji immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT hatakeyamajunko immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT okakyoko immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT tsurugaeichi immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT inaitetsuichiro immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT ananhisashi immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage AT sawayoshihiko immunohistochemicalstudyofamelogeninandlysosomeassociatemembraneproteinslampsincartilage
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Immunohistochemical Study of Amelogenin and Lysosome-Associate Membrane Proteins (LAMPs) in Cartilage

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