Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats

Metformin, an oral biguanide approved for the treatment of type II diabetes is widely prescribed for other clinical conditions. Currently, metformin is being investigated as potential anti-tumor agent. However, there have been recent concerns about hepatotoxicity associated with the use of metformin...

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Autor principal:	Gharbaran,Rajendra
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Metformin Rats TEM Cancer Diabetes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300016
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spelling	oai:scielo:S0717-950220140003000162015-11-16Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive RatsGharbaran,Rajendra Metformin Rats TEM Cancer Diabetes Metformin, an oral biguanide approved for the treatment of type II diabetes is widely prescribed for other clinical conditions. Currently, metformin is being investigated as potential anti-tumor agent. However, there have been recent concerns about hepatotoxicity associated with the use of metformin. This study, by means of high resolution transmission electron microscopy (TEM) and morphometry, investigated potential ultrastructural changes induced by metformin treatment on the hepatocytes of spontaneously hypertensive rats (SHR). Morphometric analysis was carried out on images of randomly selected cells from sectioned gluteraldehyde-osmium-fixed, Epon embedded liver tissue. One-way analysis of variance (ANOVA) on morphometric data showed statistically significant differences in the mean volume density (MVD) of lipid bodies (F=136.48, P<0.0001)and mean surface density (MSD) of endoplasmic reticulum (ER) (F=12.45, P<0.003) between hepatocytes of control (n=8) and metformin-treated (MT) (n=8) animals. MVD for control group was 5.42% (±0.36 SEM) but decreased significantly in the MT group (1.13%, ±0.04 SEM). Similarly, MSD of ER for control was 24.7 µm2/µm3 (±1.64 SEM) but decreased for MT animals (18.90 µm2/µm3, ±0.28 SEM). These data are most likely consistent with the effects of metformin on lipid metabolism, and may not reflect on hepatotoxicity induced by the drug, in SHRs.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.3 20142014-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300016en10.4067/S0717-95022014000300016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Metformin Rats TEM Cancer Diabetes
spellingShingle	Metformin Rats TEM Cancer Diabetes Gharbaran,Rajendra Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
description	Metformin, an oral biguanide approved for the treatment of type II diabetes is widely prescribed for other clinical conditions. Currently, metformin is being investigated as potential anti-tumor agent. However, there have been recent concerns about hepatotoxicity associated with the use of metformin. This study, by means of high resolution transmission electron microscopy (TEM) and morphometry, investigated potential ultrastructural changes induced by metformin treatment on the hepatocytes of spontaneously hypertensive rats (SHR). Morphometric analysis was carried out on images of randomly selected cells from sectioned gluteraldehyde-osmium-fixed, Epon embedded liver tissue. One-way analysis of variance (ANOVA) on morphometric data showed statistically significant differences in the mean volume density (MVD) of lipid bodies (F=136.48, P<0.0001)and mean surface density (MSD) of endoplasmic reticulum (ER) (F=12.45, P<0.003) between hepatocytes of control (n=8) and metformin-treated (MT) (n=8) animals. MVD for control group was 5.42% (±0.36 SEM) but decreased significantly in the MT group (1.13%, ±0.04 SEM). Similarly, MSD of ER for control was 24.7 µm2/µm3 (±1.64 SEM) but decreased for MT animals (18.90 µm2/µm3, ±0.28 SEM). These data are most likely consistent with the effects of metformin on lipid metabolism, and may not reflect on hepatotoxicity induced by the drug, in SHRs.
author	Gharbaran,Rajendra
author_facet	Gharbaran,Rajendra
author_sort	Gharbaran,Rajendra
title	Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
title_short	Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
title_full	Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
title_fullStr	Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
title_full_unstemmed	Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats
title_sort	metformin induces ultrastructural alterations in hepatocytes of spontaneously hypertensive rats
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300016
work_keys_str_mv	AT gharbaranrajendra metformininducesultrastructuralalterationsinhepatocytesofspontaneouslyhypertensiverats
_version_	1718444879605923840

Metformin Induces Ultrastructural Alterations in Hepatocytes of Spontaneously Hypertensive Rats

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