Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes

Previous studies have shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal models. This study was conducted to determine the effect of gestational diabetes on retinal ganglionic cell density, the thickness of the retinal layer and apoptotic ganglionic cell dens...

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Autores principales:	Najafdari,Simin, Rezaei,Noorallah, Shafaroodi,Majid Malekzadeh, Ghafari,Soraya, Golalipour,Mohammad Jafar
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Gestational diabetes Apoptosis Retinal layer Ganglionic cells TUNEL Assay Rat offspring
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400001
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spelling	oai:scielo:S0717-950220140004000012015-11-16Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational DiabetesNajafdari,SiminRezaei,NoorallahShafaroodi,Majid MalekzadehGhafari,SorayaGolalipour,Mohammad Jafar Gestational diabetes Apoptosis Retinal layer Ganglionic cells TUNEL Assay Rat offspring Previous studies have shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal models. This study was conducted to determine the effect of gestational diabetes on retinal ganglionic cell density, the thickness of the retinal layer and apoptotic ganglionic cell density in 28-day-old of rat offspring. In this experimental study, 10 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally, dams in control group received an equivalent volume normal saline. At postnatal day 28, six offspring of each gestational diabetes and controls were randomly selected, sacrificed and sections (6 micrometer) were taken from the eye and stained with hematoxylin and eosin. The density of ganglionic cells and the number of dUTP end-labeling (TUNEL)-positive cells were evaluated in 20000 mm2 area of ganglion layer of the retina. The ganglionic cells density were reduced (27.4%) in gestational diabetic offspring in compared to controls (22.5±1.5 vs. 31.0±0.9, P<0.01). The apoptotic ganglionic cells of retina in interventional group significantly increased in compared to controls (6.74±0.60 vs. 5.12±0.26, P<0.02). This study showed that the uncontrolled gestational diabetes can reduce the number of ganglionic cells and increase apoptotic ganglionic cells of retina layer in rat offspring.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400001en10.4067/S0717-95022014000400001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gestational diabetes Apoptosis Retinal layer Ganglionic cells TUNEL Assay Rat offspring
spellingShingle	Gestational diabetes Apoptosis Retinal layer Ganglionic cells TUNEL Assay Rat offspring Najafdari,Simin Rezaei,Noorallah Shafaroodi,Majid Malekzadeh Ghafari,Soraya Golalipour,Mohammad Jafar Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
description	Previous studies have shown the adverse effects of gestational diabetes on hippocampal neuronal density in animal models. This study was conducted to determine the effect of gestational diabetes on retinal ganglionic cell density, the thickness of the retinal layer and apoptotic ganglionic cell density in 28-day-old of rat offspring. In this experimental study, 10 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally, dams in control group received an equivalent volume normal saline. At postnatal day 28, six offspring of each gestational diabetes and controls were randomly selected, sacrificed and sections (6 micrometer) were taken from the eye and stained with hematoxylin and eosin. The density of ganglionic cells and the number of dUTP end-labeling (TUNEL)-positive cells were evaluated in 20000 mm2 area of ganglion layer of the retina. The ganglionic cells density were reduced (27.4%) in gestational diabetic offspring in compared to controls (22.5±1.5 vs. 31.0±0.9, P<0.01). The apoptotic ganglionic cells of retina in interventional group significantly increased in compared to controls (6.74±0.60 vs. 5.12±0.26, P<0.02). This study showed that the uncontrolled gestational diabetes can reduce the number of ganglionic cells and increase apoptotic ganglionic cells of retina layer in rat offspring.
author	Najafdari,Simin Rezaei,Noorallah Shafaroodi,Majid Malekzadeh Ghafari,Soraya Golalipour,Mohammad Jafar
author_facet	Najafdari,Simin Rezaei,Noorallah Shafaroodi,Majid Malekzadeh Ghafari,Soraya Golalipour,Mohammad Jafar
author_sort	Najafdari,Simin
title	Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
title_short	Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
title_full	Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
title_fullStr	Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
title_full_unstemmed	Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes
title_sort	ganglionic cells apoptosis in retinal layer of rat offspring due to gestational diabetes
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400001
work_keys_str_mv	AT najafdarisimin ganglioniccellsapoptosisinretinallayerofratoffspringduetogestationaldiabetes AT rezaeinoorallah ganglioniccellsapoptosisinretinallayerofratoffspringduetogestationaldiabetes AT shafaroodimajidmalekzadeh ganglioniccellsapoptosisinretinallayerofratoffspringduetogestationaldiabetes AT ghafarisoraya ganglioniccellsapoptosisinretinallayerofratoffspringduetogestationaldiabetes AT golalipourmohammadjafar ganglioniccellsapoptosisinretinallayerofratoffspringduetogestationaldiabetes
_version_	1718444887982997504

Ganglionic Cells Apoptosis in Retinal Layer of Rat Offspring due to Gestational Diabetes

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