Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods

Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to eval...

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Autores principales:	Millas,Ieda, Barros,Mirna Duarte, Curcio,Daniella Franco, Buck,Hudson Sousa, Liquidato,Bianca Maria
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2014
Materias:	Immunohistochemistry Estrogen receptors Methods Microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007
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spelling	oai:scielo:S0717-950220140004000072015-11-16Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation MethodsMillas,IedaBarros,Mirna DuarteCurcio,Daniella FrancoBuck,Hudson SousaLiquidato,Bianca Maria Immunohistochemistry Estrogen receptors Methods Microscopy Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density (ROD) reading, through the MCID 7.0 system of densitometric digital analysis (Inter Focus Imaging LTDA, Linton, England). Subjective and objective classifications were compared under statistical analysis (Kolmogorov-Smirnov and Spearman Correlation Test, p<0.05). We found a positive correlation between the subjective findings of observers and digital analysis in all the categories of beta receptor. For the alpha receptor, there was a correlation only in extreme categories. The subjective evaluations by observers and digital method by measuring the relative optical density show statistically significant correlations in quantifying the estrogen receptors by immunohistochemistry staining. This indicates that both methods show accuracy for the proposed study.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.32 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007en10.4067/S0717-95022014000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Immunohistochemistry Estrogen receptors Methods Microscopy
spellingShingle	Immunohistochemistry Estrogen receptors Methods Microscopy Millas,Ieda Barros,Mirna Duarte Curcio,Daniella Franco Buck,Hudson Sousa Liquidato,Bianca Maria Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
description	Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density (ROD) reading, through the MCID 7.0 system of densitometric digital analysis (Inter Focus Imaging LTDA, Linton, England). Subjective and objective classifications were compared under statistical analysis (Kolmogorov-Smirnov and Spearman Correlation Test, p<0.05). We found a positive correlation between the subjective findings of observers and digital analysis in all the categories of beta receptor. For the alpha receptor, there was a correlation only in extreme categories. The subjective evaluations by observers and digital method by measuring the relative optical density show statistically significant correlations in quantifying the estrogen receptors by immunohistochemistry staining. This indicates that both methods show accuracy for the proposed study.
author	Millas,Ieda Barros,Mirna Duarte Curcio,Daniella Franco Buck,Hudson Sousa Liquidato,Bianca Maria
author_facet	Millas,Ieda Barros,Mirna Duarte Curcio,Daniella Franco Buck,Hudson Sousa Liquidato,Bianca Maria
author_sort	Millas,Ieda
title	Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
title_short	Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
title_full	Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
title_fullStr	Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
title_full_unstemmed	Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
title_sort	estrogen receptors in immunohistochemically stained slides: comparing subjective and computerized evaluation methods
publisher	Sociedad Chilena de Anatomía
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007
work_keys_str_mv	AT millasieda estrogenreceptorsinimmunohistochemicallystainedslidescomparingsubjectiveandcomputerizedevaluationmethods AT barrosmirnaduarte estrogenreceptorsinimmunohistochemicallystainedslidescomparingsubjectiveandcomputerizedevaluationmethods AT curciodaniellafranco estrogenreceptorsinimmunohistochemicallystainedslidescomparingsubjectiveandcomputerizedevaluationmethods AT buckhudsonsousa estrogenreceptorsinimmunohistochemicallystainedslidescomparingsubjectiveandcomputerizedevaluationmethods AT liquidatobiancamaria estrogenreceptorsinimmunohistochemicallystainedslidescomparingsubjectiveandcomputerizedevaluationmethods
_version_	1718444889106022400

Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods

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