The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats
The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induce...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2015
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oai:scielo:S0717-950220150001000312015-04-09The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced RatsYang,ZhiXiao,KeWang,WeiTang,JuanSun,Peng-PengPeng,Ke-MeiSong,Hui Visfatin LPS-induced rat Uterus Inflammation Cytokine The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat were studied using hematoxylin-eosin staining (HE), immunohistochemical methods, western blots and enzyme-linked immunosorbent assay (ELISA). The present study showed that visfatin positive cells dispersed widely in the uterus, and strong positive staining was observed mainly in the cell cytoplasm. Compared with saline group, in visfatin group, more uterine glands were found, EOS increased, and the difference was significant (P<0.05), MPO reduced, and the difference was significant (P<0.01). In addition, visfatin was able to increase the secretion of IL-1b, IL-6, and TNF-a (P<0.01). Compared with LPS group, in vifatin+LPS group, the uterine glands of the lamina propria increased, the myometrium became thinner, the number of EOS and MPO reduced obviously, but the difference was not significant (P>0.05), and after LPS stimulated body, visfatin decrease the level of IL-1b, IL-6, TNF-a (P<0.01). The above results suggest that visfatin could affect the morphological structure of rat uterus; Visfatin could modulate the inflammatory response in rats' uterus by regulating the quantity of inflammatory cells, such as EOS and MPO, and the level of inflammatory cytokines, such as IL-1b, IL-6, TNF-a.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.1 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100031en10.4067/S0717-95022015000100031 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Visfatin LPS-induced rat Uterus Inflammation Cytokine |
| spellingShingle |
Visfatin LPS-induced rat Uterus Inflammation Cytokine Yang,Zhi Xiao,Ke Wang,Wei Tang,Juan Sun,Peng-Peng Peng,Ke-Mei Song,Hui The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| description |
The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat were studied using hematoxylin-eosin staining (HE), immunohistochemical methods, western blots and enzyme-linked immunosorbent assay (ELISA). The present study showed that visfatin positive cells dispersed widely in the uterus, and strong positive staining was observed mainly in the cell cytoplasm. Compared with saline group, in visfatin group, more uterine glands were found, EOS increased, and the difference was significant (P<0.05), MPO reduced, and the difference was significant (P<0.01). In addition, visfatin was able to increase the secretion of IL-1b, IL-6, and TNF-a (P<0.01). Compared with LPS group, in vifatin+LPS group, the uterine glands of the lamina propria increased, the myometrium became thinner, the number of EOS and MPO reduced obviously, but the difference was not significant (P>0.05), and after LPS stimulated body, visfatin decrease the level of IL-1b, IL-6, TNF-a (P<0.01). The above results suggest that visfatin could affect the morphological structure of rat uterus; Visfatin could modulate the inflammatory response in rats' uterus by regulating the quantity of inflammatory cells, such as EOS and MPO, and the level of inflammatory cytokines, such as IL-1b, IL-6, TNF-a. |
| author |
Yang,Zhi Xiao,Ke Wang,Wei Tang,Juan Sun,Peng-Peng Peng,Ke-Mei Song,Hui |
| author_facet |
Yang,Zhi Xiao,Ke Wang,Wei Tang,Juan Sun,Peng-Peng Peng,Ke-Mei Song,Hui |
| author_sort |
Yang,Zhi |
| title |
The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| title_short |
The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| title_full |
The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| title_fullStr |
The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| title_full_unstemmed |
The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats |
| title_sort |
effect of visfatin on inflammatory reaction in uterus of lps-induced rats |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2015 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100031 |
| work_keys_str_mv |
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| _version_ |
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