Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice
Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoho...
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Sociedad Chilena de Anatomía
2015
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oai:scielo:S0717-950220150001000442015-04-09Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in MiceKeshtmand,ZahraGhanbari,AliKhazaei,MozafarRabzia,Arezou Cisplatin Tribulus terrestris Apoptosis TUNEL Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis and also TUNEL assay on mice testis were performed. Weights of body and testis, seminiferous tubules diameter and apoptotic index were assessed. Data analysis was performed using one-way ANOVA followed by Tukeys' test. The results showed that cisplatin lead to a reduction in the weight of body and testes, and significantly increased apoptotic index compared to the control group (P<0.001), while in treated groups with TT, the weights of body and testis and seminiferous tubules diameter were significantly higher compared with cisplatin group (P<0.001), but apoptotic index did not show significant differences. The study demonstrates that extract of TT could protective effect of on cisplatin-induced apoptosis of testis and seminiferous tubules diameter that may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.1 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100044en10.4067/S0717-95022015000100044 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Cisplatin Tribulus terrestris Apoptosis TUNEL |
| spellingShingle |
Cisplatin Tribulus terrestris Apoptosis TUNEL Keshtmand,Zahra Ghanbari,Ali Khazaei,Mozafar Rabzia,Arezou Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| description |
Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis and also TUNEL assay on mice testis were performed. Weights of body and testis, seminiferous tubules diameter and apoptotic index were assessed. Data analysis was performed using one-way ANOVA followed by Tukeys' test. The results showed that cisplatin lead to a reduction in the weight of body and testes, and significantly increased apoptotic index compared to the control group (P<0.001), while in treated groups with TT, the weights of body and testis and seminiferous tubules diameter were significantly higher compared with cisplatin group (P<0.001), but apoptotic index did not show significant differences. The study demonstrates that extract of TT could protective effect of on cisplatin-induced apoptosis of testis and seminiferous tubules diameter that may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms. |
| author |
Keshtmand,Zahra Ghanbari,Ali Khazaei,Mozafar Rabzia,Arezou |
| author_facet |
Keshtmand,Zahra Ghanbari,Ali Khazaei,Mozafar Rabzia,Arezou |
| author_sort |
Keshtmand,Zahra |
| title |
Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| title_short |
Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| title_full |
Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| title_fullStr |
Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| title_full_unstemmed |
Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice |
| title_sort |
protective effect of tribulus terrestris hydroalcoholic extract against cisplatininduced apoptosis on testis in mice |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2015 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100044 |
| work_keys_str_mv |
AT keshtmandzahra protectiveeffectoftribulusterrestrishydroalcoholicextractagainstcisplatininducedapoptosisontestisinmice AT ghanbariali protectiveeffectoftribulusterrestrishydroalcoholicextractagainstcisplatininducedapoptosisontestisinmice AT khazaeimozafar protectiveeffectoftribulusterrestrishydroalcoholicextractagainstcisplatininducedapoptosisontestisinmice AT rabziaarezou protectiveeffectoftribulusterrestrishydroalcoholicextractagainstcisplatininducedapoptosisontestisinmice |
| _version_ |
1718444906951737344 |