Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)

Ecstasy is one of the most popular amusing drugs among young people. Documents indicate some effects of Ecstasy on hippocampus and close relations between dopaminergic functions with reward learning. Therefore, the aim of this study was evaluation of the chronic effects of Ecstasy on memory in male...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simin,Mahakizadeh, Mehrdad,Jahanshahi, Kamran,Haidari, Majid,Shahbazi
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	MDMA Dopamine Receptors Gene expression Hippocampus Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100048
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022015000100048
record_format	dspace
spelling	oai:scielo:S0717-950220150001000482015-04-09Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)Simin,MahakizadehMehrdad,JahanshahiKamran,HaidariMajid,Shahbazi MDMA Dopamine Receptors Gene expression Hippocampus Rat Ecstasy is one of the most popular amusing drugs among young people. Documents indicate some effects of Ecstasy on hippocampus and close relations between dopaminergic functions with reward learning. Therefore, the aim of this study was evaluation of the chronic effects of Ecstasy on memory in male Wistar rats and determination of dopamine receptors' gene expression in hippocampus. Forty adult male Wistar rats randomly distributed in five groups: Control, sham (received 1 ml/kg 0.9% saline) and three experimental groups were: Exp. 1 (2.5 mg/kg), Exp. 2 (5 mg/kg), and Exp. 3 (10 mg/kg) received MDMA intraperitoneally once every 7 days (3 times a day, 3 hours apart) for 4 weeks. Before the first injection animals trained in Shuttle Box memory and tested after the last injection. 24 hours after the final testing, brains of rats were dissected and hippocampus was removed and homogenized. After total RNA extraction and cDNA synthesis, expression of dopamine receptor genes in the hippocampus determined with Real-Time PCR. Our results showed that 2.5 and 5 mg/kg MDMA-treated groups had memory impairment. Also we found that MDMA increased the mRNA expression of dopamine receptors in hippocampus and the highest increase found in dopamine D1 receptors in the 5 mg/kg experimental group. We concluded that low doses of Ecstasy could increase Dopamine takers gene expression in hippocampus and disorder avoidance memory. But in high doses the increase in Dopamine takers gene expression was not as much as that in low doses and avoidance memory disorder was not observed.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.1 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100048en10.4067/S0717-95022015000100048
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	MDMA Dopamine Receptors Gene expression Hippocampus Rat
spellingShingle	MDMA Dopamine Receptors Gene expression Hippocampus Rat Simin,Mahakizadeh Mehrdad,Jahanshahi Kamran,Haidari Majid,Shahbazi Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
description	Ecstasy is one of the most popular amusing drugs among young people. Documents indicate some effects of Ecstasy on hippocampus and close relations between dopaminergic functions with reward learning. Therefore, the aim of this study was evaluation of the chronic effects of Ecstasy on memory in male Wistar rats and determination of dopamine receptors' gene expression in hippocampus. Forty adult male Wistar rats randomly distributed in five groups: Control, sham (received 1 ml/kg 0.9% saline) and three experimental groups were: Exp. 1 (2.5 mg/kg), Exp. 2 (5 mg/kg), and Exp. 3 (10 mg/kg) received MDMA intraperitoneally once every 7 days (3 times a day, 3 hours apart) for 4 weeks. Before the first injection animals trained in Shuttle Box memory and tested after the last injection. 24 hours after the final testing, brains of rats were dissected and hippocampus was removed and homogenized. After total RNA extraction and cDNA synthesis, expression of dopamine receptor genes in the hippocampus determined with Real-Time PCR. Our results showed that 2.5 and 5 mg/kg MDMA-treated groups had memory impairment. Also we found that MDMA increased the mRNA expression of dopamine receptors in hippocampus and the highest increase found in dopamine D1 receptors in the 5 mg/kg experimental group. We concluded that low doses of Ecstasy could increase Dopamine takers gene expression in hippocampus and disorder avoidance memory. But in high doses the increase in Dopamine takers gene expression was not as much as that in low doses and avoidance memory disorder was not observed.
author	Simin,Mahakizadeh Mehrdad,Jahanshahi Kamran,Haidari Majid,Shahbazi
author_facet	Simin,Mahakizadeh Mehrdad,Jahanshahi Kamran,Haidari Majid,Shahbazi
author_sort	Simin,Mahakizadeh
title	Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
title_short	Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
title_full	Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
title_fullStr	Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
title_full_unstemmed	Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)
title_sort	dopamine receptors gene expression in male rat hippocampus after administration of mdma (ecstasy)
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100048
work_keys_str_mv	AT siminmahakizadeh dopaminereceptorsgeneexpressioninmalerathippocampusafteradministrationofmdmaecstasy AT mehrdadjahanshahi dopaminereceptorsgeneexpressioninmalerathippocampusafteradministrationofmdmaecstasy AT kamranhaidari dopaminereceptorsgeneexpressioninmalerathippocampusafteradministrationofmdmaecstasy AT majidshahbazi dopaminereceptorsgeneexpressioninmalerathippocampusafteradministrationofmdmaecstasy
_version_	1718444907702517760

Dopamine Receptors Gene Expression in Male Rat Hippocampus after Administration of MDMA (Ecstasy)

Ejemplares similares