Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study

Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman wi...

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Autores principales:	Bohlouli,Somayeh, Khazaei,Mozafar, Rabzia,Arezou, Khazaei,Mohammad Rasool, Sadeghi,Ehsan
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Adiponectin Nitric oxide Endometriosis Stromal cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100053
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spelling	oai:scielo:S0717-950220150001000532015-04-09Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro StudyBohlouli,SomayehKhazaei,MozafarRabzia,ArezouKhazaei,Mohammad RasoolSadeghi,Ehsan Adiponectin Nitric oxide Endometriosis Stromal cell Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman with endometriosis. The aim of this study was to determine adiponectin effect on nitric oxide secretion by cultured normal and endometriotic human endometrial stromal cells. In this experimental study, normal (n= 10) and endometriotic endometrial biopsies (n= 10) were taken in sterile condition. Stromal cells isolated and cultured in in DMEM/ F12 medium and treated with adiponectin concentrations (0, 10, 100, and 200 ng/ml) for 24 and 48 hours. NO assay was done on their supernatants by Greiss method. Data was analyzed by one way ANOVA and p<0.05 was considered significant. There was significant difference between endometriosis groups in NO secretion in all dose of adiponectin and time (p<0.05). In normal groups there was significant difference in 48 hours (p<0.05) but no significant change in 24 hours (p>0.05). Adiponectin effects nitric oxide secretion of cultured human endometriotic stromal cells.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.1 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100053en10.4067/S0717-95022015000100053
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adiponectin Nitric oxide Endometriosis Stromal cell
spellingShingle	Adiponectin Nitric oxide Endometriosis Stromal cell Bohlouli,Somayeh Khazaei,Mozafar Rabzia,Arezou Khazaei,Mohammad Rasool Sadeghi,Ehsan Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
description	Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman with endometriosis. The aim of this study was to determine adiponectin effect on nitric oxide secretion by cultured normal and endometriotic human endometrial stromal cells. In this experimental study, normal (n= 10) and endometriotic endometrial biopsies (n= 10) were taken in sterile condition. Stromal cells isolated and cultured in in DMEM/ F12 medium and treated with adiponectin concentrations (0, 10, 100, and 200 ng/ml) for 24 and 48 hours. NO assay was done on their supernatants by Greiss method. Data was analyzed by one way ANOVA and p<0.05 was considered significant. There was significant difference between endometriosis groups in NO secretion in all dose of adiponectin and time (p<0.05). In normal groups there was significant difference in 48 hours (p<0.05) but no significant change in 24 hours (p>0.05). Adiponectin effects nitric oxide secretion of cultured human endometriotic stromal cells.
author	Bohlouli,Somayeh Khazaei,Mozafar Rabzia,Arezou Khazaei,Mohammad Rasool Sadeghi,Ehsan
author_facet	Bohlouli,Somayeh Khazaei,Mozafar Rabzia,Arezou Khazaei,Mohammad Rasool Sadeghi,Ehsan
author_sort	Bohlouli,Somayeh
title	Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
title_short	Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
title_full	Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
title_fullStr	Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
title_full_unstemmed	Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
title_sort	adiponectin effect on nitric oxide secretion by normal and endometriotic human endometrial stromal cells: in vitro study
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100053
work_keys_str_mv	AT bohloulisomayeh adiponectineffectonnitricoxidesecretionbynormalandendometriotichumanendometrialstromalcellsinvitrostudy AT khazaeimozafar adiponectineffectonnitricoxidesecretionbynormalandendometriotichumanendometrialstromalcellsinvitrostudy AT rabziaarezou adiponectineffectonnitricoxidesecretionbynormalandendometriotichumanendometrialstromalcellsinvitrostudy AT khazaeimohammadrasool adiponectineffectonnitricoxidesecretionbynormalandendometriotichumanendometrialstromalcellsinvitrostudy AT sadeghiehsan adiponectineffectonnitricoxidesecretionbynormalandendometriotichumanendometrialstromalcellsinvitrostudy
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Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study

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