Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice

The microtubule-associated protein MAP-2 is an integral part of the cytoskeleton and plays an important role in neural morphogenesis. This protein is an essential component of the dendritic cytoskeleton, especially in the adult brain, and its expression can be altered under experimental or pathologi...

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Autores principales:	Hurtado,Andrea P, Rengifo,Aura Caterine, Torres-Fernández,Orlando
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	MAP-2 Cerebral cortex Pyramidal neurons Rabies virus Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200010
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spelling	oai:scielo:S0717-950220150002000102015-08-13Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected MiceHurtado,Andrea PRengifo,Aura CaterineTorres-Fernández,Orlando MAP-2 Cerebral cortex Pyramidal neurons Rabies virus Immunohistochemistry The microtubule-associated protein MAP-2 is an integral part of the cytoskeleton and plays an important role in neural morphogenesis. This protein is an essential component of the dendritic cytoskeleton, especially in the adult brain, and its expression can be altered under experimental or pathological conditions. The purpose of this study was to evaluate the effect of infection with the rabies virus on MAP-2 immunoreactivity in the cerebral cortex of mice. The mice were inoculated with the rabies virus and the animals were sacrificed when the disease reached its advanced stage, together with uninfected animals of the same age. The brains were extracted after being previously perfusion-fixed with paraformaldehyde; coronal sections were obtained with a vibratome. The coronal sections were processed by immunohistochemistry to reveal the presence of the MAP-2 protein in neurons of the motor area of the cerebral cortex. Rabies-infected mice showed an increase in the immunoreactivity of the somata and apical dendrites in pyramidal neurons of the motor cortex. This is an unexpected result, as dendritic pathology has been previously demonstrated in rabies, and some studies on neurological disorders associate dendritic alterations with loss of expression of the MAP-2 protein. Therefore, whatever the alteration in the expression of this protein, decrease or increase, it could be causing a biochemical imbalance in the integrity and stability of the neuronal cytoskeleton.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.2 20152015-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200010en10.4067/S0717-95022015000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	MAP-2 Cerebral cortex Pyramidal neurons Rabies virus Immunohistochemistry
spellingShingle	MAP-2 Cerebral cortex Pyramidal neurons Rabies virus Immunohistochemistry Hurtado,Andrea P Rengifo,Aura Caterine Torres-Fernández,Orlando Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
description	The microtubule-associated protein MAP-2 is an integral part of the cytoskeleton and plays an important role in neural morphogenesis. This protein is an essential component of the dendritic cytoskeleton, especially in the adult brain, and its expression can be altered under experimental or pathological conditions. The purpose of this study was to evaluate the effect of infection with the rabies virus on MAP-2 immunoreactivity in the cerebral cortex of mice. The mice were inoculated with the rabies virus and the animals were sacrificed when the disease reached its advanced stage, together with uninfected animals of the same age. The brains were extracted after being previously perfusion-fixed with paraformaldehyde; coronal sections were obtained with a vibratome. The coronal sections were processed by immunohistochemistry to reveal the presence of the MAP-2 protein in neurons of the motor area of the cerebral cortex. Rabies-infected mice showed an increase in the immunoreactivity of the somata and apical dendrites in pyramidal neurons of the motor cortex. This is an unexpected result, as dendritic pathology has been previously demonstrated in rabies, and some studies on neurological disorders associate dendritic alterations with loss of expression of the MAP-2 protein. Therefore, whatever the alteration in the expression of this protein, decrease or increase, it could be causing a biochemical imbalance in the integrity and stability of the neuronal cytoskeleton.
author	Hurtado,Andrea P Rengifo,Aura Caterine Torres-Fernández,Orlando
author_facet	Hurtado,Andrea P Rengifo,Aura Caterine Torres-Fernández,Orlando
author_sort	Hurtado,Andrea P
title	Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
title_short	Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
title_full	Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
title_fullStr	Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
title_full_unstemmed	Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice
title_sort	immunohistochemical overexpression of map-2 in the cerebral cortex of rabies-infected mice
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200010
work_keys_str_mv	AT hurtadoandreap immunohistochemicaloverexpressionofmap2inthecerebralcortexofrabiesinfectedmice AT rengifoauracaterine immunohistochemicaloverexpressionofmap2inthecerebralcortexofrabiesinfectedmice AT torresfernandezorlando immunohistochemicaloverexpressionofmap2inthecerebralcortexofrabiesinfectedmice
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Immunohistochemical Overexpression of MAP-2 in the Cerebral Cortex of Rabies-Infected Mice

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