Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines

Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell...

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Autores principales:	Ili,Carmen G, Brebi,Priscilla, Garcia,Patricia, Leal,Pamela, Lopez,Jaime, Tapia,Oscar, Letelier,Pablo, Weber,Helga, Castillo,Jonathan, Roa,Juan C
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Apoptosis Proliferation Cervical cancer siRNA c-FLIPL
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036
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spelling	oai:scielo:S0717-950220150002000362015-08-13Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell LinesIli,Carmen GBrebi,PriscillaGarcia,PatriciaLeal,PamelaLopez,JaimeTapia,OscarLetelier,PabloWeber,HelgaCastillo,JonathanRoa,Juan C Apoptosis Proliferation Cervical cancer siRNA c-FLIPL Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P<0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P<0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P<0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.2 20152015-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036en10.4067/S0717-95022015000200036
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Apoptosis Proliferation Cervical cancer siRNA c-FLIPL
spellingShingle	Apoptosis Proliferation Cervical cancer siRNA c-FLIPL Ili,Carmen G Brebi,Priscilla Garcia,Patricia Leal,Pamela Lopez,Jaime Tapia,Oscar Letelier,Pablo Weber,Helga Castillo,Jonathan Roa,Juan C Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
description	Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P<0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P<0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P<0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.
author	Ili,Carmen G Brebi,Priscilla Garcia,Patricia Leal,Pamela Lopez,Jaime Tapia,Oscar Letelier,Pablo Weber,Helga Castillo,Jonathan Roa,Juan C
author_facet	Ili,Carmen G Brebi,Priscilla Garcia,Patricia Leal,Pamela Lopez,Jaime Tapia,Oscar Letelier,Pablo Weber,Helga Castillo,Jonathan Roa,Juan C
author_sort	Ili,Carmen G
title	Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
title_short	Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
title_full	Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
title_fullStr	Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
title_full_unstemmed	Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
title_sort	effects of c-flipl knockdown in cervical uterine carcinoma cell lines
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036
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Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines

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