IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate

Cleft lip and palate (CLP) is a congenital anomaly characterized by the inappropriate fusion of the upper lip, alveolus, and secondary palate. This study investigated whether expression of interferon regulatory fac tor 6 (IRF6), receptor-like tyrosine kinase (RYK), and paired-box 9 (PAX9), which are...

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Autores principales:	Smane,Liene, Pilmane,Mara
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Cleft palate Interferon regulatory factor 6 Receptor-like tyrosine kinase Paired box gene 9
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200037
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spelling	oai:scielo:S0717-950220150002000372015-08-13IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft PalateSmane,LienePilmane,Mara Cleft palate Interferon regulatory factor 6 Receptor-like tyrosine kinase Paired box gene 9 Cleft lip and palate (CLP) is a congenital anomaly characterized by the inappropriate fusion of the upper lip, alveolus, and secondary palate. This study investigated whether expression of interferon regulatory fac tor 6 (IRF6), receptor-like tyrosine kinase (RYK), and paired-box 9 (PAX9), which are essential for the normal development and morphogenesis of craniofacial structures, is dysregulated in children with CLP. Oral mucosa tissue samples were obtained from patients with complete bilateral (CB) CLP (n= 19) during corrective plastic surgery and unaffected control subjects (n= 7). IRF6, RYK, and PAX9 expression was assessed by immunohistochemistry, and data were analyzed with the Mann-Whitney test. In patients, IRF6 immunoreactivity in the connective tissue was moderate to high, but the overall number of IRF6-positive oral epithelial cells was lower than that in controls (z= -3.41; P= 0.01). RYK expression was observed only sporadically in the oral epithelium of 4 patients, in contrast to the control group (z= -3.75; P< 0.001). PAX9-positive epithelial cells were present in low to moderate numbers in patients with CBCLP, while an abundance of these cells was observed in the basal layer of the oral epithelium in controls (z= -3.60; P<0.001). IRF6 is the main connective tissue regulatory factor in CBCLP, and its low level of expression in the oral epithelium suggests a reduced potential for epitheliocyte differentiation, while low PAX9 and RYK expression may explain the decreased cell migration and cleft remodeling in CBCLP.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.2 20152015-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200037en10.4067/S0717-95022015000200037
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cleft palate Interferon regulatory factor 6 Receptor-like tyrosine kinase Paired box gene 9
spellingShingle	Cleft palate Interferon regulatory factor 6 Receptor-like tyrosine kinase Paired box gene 9 Smane,Liene Pilmane,Mara IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
description	Cleft lip and palate (CLP) is a congenital anomaly characterized by the inappropriate fusion of the upper lip, alveolus, and secondary palate. This study investigated whether expression of interferon regulatory fac tor 6 (IRF6), receptor-like tyrosine kinase (RYK), and paired-box 9 (PAX9), which are essential for the normal development and morphogenesis of craniofacial structures, is dysregulated in children with CLP. Oral mucosa tissue samples were obtained from patients with complete bilateral (CB) CLP (n= 19) during corrective plastic surgery and unaffected control subjects (n= 7). IRF6, RYK, and PAX9 expression was assessed by immunohistochemistry, and data were analyzed with the Mann-Whitney test. In patients, IRF6 immunoreactivity in the connective tissue was moderate to high, but the overall number of IRF6-positive oral epithelial cells was lower than that in controls (z= -3.41; P= 0.01). RYK expression was observed only sporadically in the oral epithelium of 4 patients, in contrast to the control group (z= -3.75; P< 0.001). PAX9-positive epithelial cells were present in low to moderate numbers in patients with CBCLP, while an abundance of these cells was observed in the basal layer of the oral epithelium in controls (z= -3.60; P<0.001). IRF6 is the main connective tissue regulatory factor in CBCLP, and its low level of expression in the oral epithelium suggests a reduced potential for epitheliocyte differentiation, while low PAX9 and RYK expression may explain the decreased cell migration and cleft remodeling in CBCLP.
author	Smane,Liene Pilmane,Mara
author_facet	Smane,Liene Pilmane,Mara
author_sort	Smane,Liene
title	IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
title_short	IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
title_full	IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
title_fullStr	IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
title_full_unstemmed	IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate
title_sort	irf6, ryk, and pax9 expression in facial tissue of children with cleft palate
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200037
work_keys_str_mv	AT smaneliene irf6rykandpax9expressioninfacialtissueofchildrenwithcleftpalate AT pilmanemara irf6rykandpax9expressioninfacialtissueofchildrenwithcleftpalate
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IRF6, RYK, and PAX9 Expression in Facial Tissue of Children with Cleft Palate

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