The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats

Hypoxia-ischemia (HI) is a major cause of brain damage in the newborn. Several studies elicited the neuroprotective effects of progesterone in adult rats but there is very little literature available on neonatal rats. Therefore the present study is undertaken to see the effect of progesterone in hyp...

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Autores principales:	Khan,Aaijaz Ahmed, Ramli,Norhida Binti, Ismail,Zulizhar Mohd
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Cornu Ammonis Hippocampus Hypoxia Ischemia Neonatal rats Neuroprotective Progestrone Pyramidal cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300025
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spelling	oai:scielo:S0717-950220150003000252015-12-04The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal RatsKhan,Aaijaz AhmedRamli,Norhida BintiIsmail,Zulizhar Mohd Cornu Ammonis Hippocampus Hypoxia Ischemia Neonatal rats Neuroprotective Progestrone Pyramidal cell Hypoxia-ischemia (HI) is a major cause of brain damage in the newborn. Several studies elicited the neuroprotective effects of progesterone in adult rats but there is very little literature available on neonatal rats. Therefore the present study is undertaken to see the effect of progesterone in hypoxic ischemic brain injury in neonatal rats, using an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to right common carotid artery ligation and then 60 minutes hypoxia. The first dose of progesterone to treatment group was administered by peritoneal injection (4 mg/kg), after 10 minutes of exposure and subsequent doses were given by subcutaneous injection at 6 h, 24 h and 48 h intervals. Control group was also exposed to HI and was given only the vehicle (peanut oil) through the same route and intervals as that of treatment group. After 96 h, the pups were perfused with 10% formalin and brains were sampled and stained with toluidine blue. Cells density and number of pyramidal cells of the hippocampal Cornu Ammonis (CA) regions were examined by stereological methods. The histomorphometric assessment of the effects of progesterone showed minimal but no significant protective value in the volume, cells density and total number of pyramidal cells of hippocampal CA region of the treatment and control groups (p>0.05) after HI. Our results concluded that 4 mg/kg of PROG had no significant neuroprotective effect in HI model of the neonatal rat's hippocampus.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300025en10.4067/S0717-95022015000300025
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cornu Ammonis Hippocampus Hypoxia Ischemia Neonatal rats Neuroprotective Progestrone Pyramidal cell
spellingShingle	Cornu Ammonis Hippocampus Hypoxia Ischemia Neonatal rats Neuroprotective Progestrone Pyramidal cell Khan,Aaijaz Ahmed Ramli,Norhida Binti Ismail,Zulizhar Mohd The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
description	Hypoxia-ischemia (HI) is a major cause of brain damage in the newborn. Several studies elicited the neuroprotective effects of progesterone in adult rats but there is very little literature available on neonatal rats. Therefore the present study is undertaken to see the effect of progesterone in hypoxic ischemic brain injury in neonatal rats, using an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to right common carotid artery ligation and then 60 minutes hypoxia. The first dose of progesterone to treatment group was administered by peritoneal injection (4 mg/kg), after 10 minutes of exposure and subsequent doses were given by subcutaneous injection at 6 h, 24 h and 48 h intervals. Control group was also exposed to HI and was given only the vehicle (peanut oil) through the same route and intervals as that of treatment group. After 96 h, the pups were perfused with 10% formalin and brains were sampled and stained with toluidine blue. Cells density and number of pyramidal cells of the hippocampal Cornu Ammonis (CA) regions were examined by stereological methods. The histomorphometric assessment of the effects of progesterone showed minimal but no significant protective value in the volume, cells density and total number of pyramidal cells of hippocampal CA region of the treatment and control groups (p>0.05) after HI. Our results concluded that 4 mg/kg of PROG had no significant neuroprotective effect in HI model of the neonatal rat's hippocampus.
author	Khan,Aaijaz Ahmed Ramli,Norhida Binti Ismail,Zulizhar Mohd
author_facet	Khan,Aaijaz Ahmed Ramli,Norhida Binti Ismail,Zulizhar Mohd
author_sort	Khan,Aaijaz Ahmed
title	The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
title_short	The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
title_full	The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
title_fullStr	The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
title_full_unstemmed	The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats
title_sort	effects of progesterone on hypoxic ischemic injuries in the cornu ammonis (ca) region of the hippocampus of neonatal rats
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300025
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The Effects of Progesterone on Hypoxic Ischemic Injuries in the Cornu Ammonis (CA) Region of the Hippocampus of Neonatal Rats

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