Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats

The expression of MuRF1 and MAFbx in a denervated muscle has previously been studied. However, the expression of MuRF1 and MAFbx in the recipient and donor muscles after muscle transfer for reconstruction of joint function has not been sufficiently investigated. Forty-two adult Sprague-Dawley rats w...

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Autores principales:	Yang,Shengbo, Yue,Ning, Zeng,Xueqing
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Musculocutaneous nerve injury MuRF1 MAFbx Pectoralis major Biceps Brachii
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300027
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spelling	oai:scielo:S0717-950220150003000272015-12-04Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in RatsYang,ShengboYue,NingZeng,Xueqing Musculocutaneous nerve injury MuRF1 MAFbx Pectoralis major Biceps Brachii The expression of MuRF1 and MAFbx in a denervated muscle has previously been studied. However, the expression of MuRF1 and MAFbx in the recipient and donor muscles after muscle transfer for reconstruction of joint function has not been sufficiently investigated. Forty-two adult Sprague-Dawley rats were divided into 7 groups: normal, 1 w post-, 2 w post-, and 4 w post-musculocutaneous nerve transection; and 1 w post-, 2 w post-, and 4 w post-reconstruction of elbow flexion. Muscle wet weights were assessed, and MuRF1 and MAFbx mRNA expressions were detected by polymerase chain reaction. The length of the oblique part of the pectoralis major of an SD rat is sufficient for suture to the insertion of the biceps brachii tendon. The muscle wet weight and the wet weight retention rate of the biceps brachii continued to decline after musculocutaneous nerve transection and a gradual increase was noted after the oblique part of the pectoralis major was transferred for reconstruction of elbow flexion. The oblique part of the pectoralis major showed a decrease of only 26%. The upregulated expression of MuRF1 and MAFbx in the biceps brachii reached a peak 2 w after denervation and 1 w after elbow flexion reconstruction, with an increase of 15% and 4%, respectively. This was followed by downregulation; however, the expression had not normalized at postoperative 4 w. The increased expression of MuRF1 (17%) and MAFbx (1%) in the oblique part of the pectoralis major at postoperative 1 w had decreased to below normal levels at postoperative 4 w. The transfer of the oblique part of the pectoralis major for elbow flexion reconstruction after musculocutaneous nerve transection can downregulate the expression of MuRF1 and MAFbx in the recipient muscle and causes only transient damage to the donor muscle in rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300027en10.4067/S0717-95022015000300027
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Musculocutaneous nerve injury MuRF1 MAFbx Pectoralis major Biceps Brachii
spellingShingle	Musculocutaneous nerve injury MuRF1 MAFbx Pectoralis major Biceps Brachii Yang,Shengbo Yue,Ning Zeng,Xueqing Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
description	The expression of MuRF1 and MAFbx in a denervated muscle has previously been studied. However, the expression of MuRF1 and MAFbx in the recipient and donor muscles after muscle transfer for reconstruction of joint function has not been sufficiently investigated. Forty-two adult Sprague-Dawley rats were divided into 7 groups: normal, 1 w post-, 2 w post-, and 4 w post-musculocutaneous nerve transection; and 1 w post-, 2 w post-, and 4 w post-reconstruction of elbow flexion. Muscle wet weights were assessed, and MuRF1 and MAFbx mRNA expressions were detected by polymerase chain reaction. The length of the oblique part of the pectoralis major of an SD rat is sufficient for suture to the insertion of the biceps brachii tendon. The muscle wet weight and the wet weight retention rate of the biceps brachii continued to decline after musculocutaneous nerve transection and a gradual increase was noted after the oblique part of the pectoralis major was transferred for reconstruction of elbow flexion. The oblique part of the pectoralis major showed a decrease of only 26%. The upregulated expression of MuRF1 and MAFbx in the biceps brachii reached a peak 2 w after denervation and 1 w after elbow flexion reconstruction, with an increase of 15% and 4%, respectively. This was followed by downregulation; however, the expression had not normalized at postoperative 4 w. The increased expression of MuRF1 (17%) and MAFbx (1%) in the oblique part of the pectoralis major at postoperative 1 w had decreased to below normal levels at postoperative 4 w. The transfer of the oblique part of the pectoralis major for elbow flexion reconstruction after musculocutaneous nerve transection can downregulate the expression of MuRF1 and MAFbx in the recipient muscle and causes only transient damage to the donor muscle in rats.
author	Yang,Shengbo Yue,Ning Zeng,Xueqing
author_facet	Yang,Shengbo Yue,Ning Zeng,Xueqing
author_sort	Yang,Shengbo
title	Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
title_short	Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
title_full	Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
title_fullStr	Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
title_full_unstemmed	Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats
title_sort	expression of murf1 and mafbx in donor and recipient muscles after musculocutaneous nerve transection and partial pectoralis major muscle transfer for reconstruction of elbow flexion in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300027
work_keys_str_mv	AT yangshengbo expressionofmurf1andmafbxindonorandrecipientmusclesaftermusculocutaneousnervetransectionandpartialpectoralismajormuscletransferforreconstructionofelbowflexioninrats AT yuening expressionofmurf1andmafbxindonorandrecipientmusclesaftermusculocutaneousnervetransectionandpartialpectoralismajormuscletransferforreconstructionofelbowflexioninrats AT zengxueqing expressionofmurf1andmafbxindonorandrecipientmusclesaftermusculocutaneousnervetransectionandpartialpectoralismajormuscletransferforreconstructionofelbowflexioninrats
_version_	1718444926429036544

Expression of MuRF1 and MAFbx in Donor and Recipient Muscles after Musculocutaneous Nerve Transection and Partial Pectoralis Major Muscle Transfer for Reconstruction of Elbow Flexion in Rats

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