Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes

Obesity has reached epidemic proportions, the World Health Organization (WHO) estimates that there are more than 1,000 million overweight adults world-wide. Furthermore, obesity is characterized as an overgrowth of white adipose tissue as a result of adipocyte hypertrophy and hyperplasia. Mitochondr...

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Autores principales:	Noriega-González,J. E, Chirino,Y. I, Mata-Miranda,M. M, Vázquez-Zapién,G. J, Sánchez-Monroy,V
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Preadipocytes Differentiated mature adipocyte Mitochondrial activity Resveratrol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300044
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spelling	oai:scielo:S0717-950220150003000442015-12-04Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature AdipocytesNoriega-González,J. EChirino,Y. IMata-Miranda,M. MVázquez-Zapién,G. JSánchez-Monroy,V Preadipocytes Differentiated mature adipocyte Mitochondrial activity Resveratrol Obesity has reached epidemic proportions, the World Health Organization (WHO) estimates that there are more than 1,000 million overweight adults world-wide. Furthermore, obesity is characterized as an overgrowth of white adipose tissue as a result of adipocyte hypertrophy and hyperplasia. Mitochondria is considered the source of energy within the adipocyte, since it contains the molecular machinery, and it is involved in a large number of metabolic pathways, besides the transformation of chemical energy into adenosine triphosphate. Mitochondria shortage and adipocyte dysfunction result in an excessive accumulation of triacylglycerol in the cytoplasm, which determines an imbalance between energy production and energy expenditure. Resveratrol (RSV) is a polyphenol found in different plants and its effects have been associated with mitochondrial biogenesis. An adipogenesis in vitro model (3T3-L1 preadipocytes) was used, and these cells were differentiated into mature adipocytes. Subsequently the effect of RSV on the adipocytes morphology, the lipid content and mitochondrial activity was evaluated using microscopic and flow cytometry techniques. The effect of RSV on differentiated mature adipocytes, was characterized by the decrease in lipid content and the consequently declination of the mitochondrial activity. 3T3-L1 preadipocytes retained the differentiation ability until passage 18. The RSV at doses of 25 and 50 µM for 48 hours in differentiated mature adipocytes promoted the decreased in lipid content probably due to an increase in mitochondrial activity in the early hours of RSV exposure, causing the consequently declination of mitochondrial activity at the end of 48 hours.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300044en10.4067/S0717-95022015000300044
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Preadipocytes Differentiated mature adipocyte Mitochondrial activity Resveratrol
spellingShingle	Preadipocytes Differentiated mature adipocyte Mitochondrial activity Resveratrol Noriega-González,J. E Chirino,Y. I Mata-Miranda,M. M Vázquez-Zapién,G. J Sánchez-Monroy,V Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
description	Obesity has reached epidemic proportions, the World Health Organization (WHO) estimates that there are more than 1,000 million overweight adults world-wide. Furthermore, obesity is characterized as an overgrowth of white adipose tissue as a result of adipocyte hypertrophy and hyperplasia. Mitochondria is considered the source of energy within the adipocyte, since it contains the molecular machinery, and it is involved in a large number of metabolic pathways, besides the transformation of chemical energy into adenosine triphosphate. Mitochondria shortage and adipocyte dysfunction result in an excessive accumulation of triacylglycerol in the cytoplasm, which determines an imbalance between energy production and energy expenditure. Resveratrol (RSV) is a polyphenol found in different plants and its effects have been associated with mitochondrial biogenesis. An adipogenesis in vitro model (3T3-L1 preadipocytes) was used, and these cells were differentiated into mature adipocytes. Subsequently the effect of RSV on the adipocytes morphology, the lipid content and mitochondrial activity was evaluated using microscopic and flow cytometry techniques. The effect of RSV on differentiated mature adipocytes, was characterized by the decrease in lipid content and the consequently declination of the mitochondrial activity. 3T3-L1 preadipocytes retained the differentiation ability until passage 18. The RSV at doses of 25 and 50 µM for 48 hours in differentiated mature adipocytes promoted the decreased in lipid content probably due to an increase in mitochondrial activity in the early hours of RSV exposure, causing the consequently declination of mitochondrial activity at the end of 48 hours.
author	Noriega-González,J. E Chirino,Y. I Mata-Miranda,M. M Vázquez-Zapién,G. J Sánchez-Monroy,V
author_facet	Noriega-González,J. E Chirino,Y. I Mata-Miranda,M. M Vázquez-Zapién,G. J Sánchez-Monroy,V
author_sort	Noriega-González,J. E
title	Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
title_short	Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
title_full	Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
title_fullStr	Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
title_full_unstemmed	Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes
title_sort	effect of resveratrol on mitochondrial activity in differentiated mature adipocytes
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300044
work_keys_str_mv	AT noriegagonzalezje effectofresveratrolonmitochondrialactivityindifferentiatedmatureadipocytes AT chirinoyi effectofresveratrolonmitochondrialactivityindifferentiatedmatureadipocytes AT matamirandamm effectofresveratrolonmitochondrialactivityindifferentiatedmatureadipocytes AT vazquezzapiengj effectofresveratrolonmitochondrialactivityindifferentiatedmatureadipocytes AT sanchezmonroyv effectofresveratrolonmitochondrialactivityindifferentiatedmatureadipocytes
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Effect of Resveratrol on Mitochondrial Activity in Differentiated Mature Adipocytes

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