Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets

Microfold (M) cells act as antigen-sampling sites for initiating antigen specific mucosal immune responses, but they may also provide a gateway for enteropathogen entry. In this study we demonstrated villous M cells by morphological and immunohistochemical methods to be present in the three regions...

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Autores principales:	Renfeng,Li, Xiangqin,Tian, Songlin,Qiao, Yanyan,Yang, Enmin,Zhou, Gaiping,Zhang
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	M Cells Villous epithelium Small intestine Newborn piglets
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400011
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spelling	oai:scielo:S0717-950220150004000112016-03-22Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn PigletsRenfeng,LiXiangqin,TianSonglin,QiaoYanyan,YangEnmin,ZhouGaiping,Zhang M Cells Villous epithelium Small intestine Newborn piglets Microfold (M) cells act as antigen-sampling sites for initiating antigen specific mucosal immune responses, but they may also provide a gateway for enteropathogen entry. In this study we demonstrated villous M cells by morphological and immunohistochemical methods to be present in the three regions of the small intestine from newborn piglets. Immunohistochemical analysis, using anti- cytokeratin 18 (CK18) primary antibodies, showed a gradually decreased density of M cells from the lower crypt epithelium to the upper villous epithelium. The proportion of villous M cells was greater in the ileum than in the duodenum or the mid-jejunum. Ultrastructural observation revealed that villous M cells were mainly columnar in shape in the duodenum and the mid-jejunum, and appeared as more pocket-like structure in the ileum. These villous M cells exhibited short and irregular microvilli, rich vesicles and reduced glycocalyx, but lacked the lymphocyte-containing basolateral invagination. Our results support evidence that M cells can develop in the small intestinal villous epithelium of newborn piglets, implying that villous M cells may begin developing in the pig's small intestine during fetal stages, which depends neither on the influence of the mucosal lymphoid tissue nor the antigen from the intestinal lumen stimulation. In addition, the variable morphology and heterogeneity distribution of villous M cells in the three regions of the small intestine may be indicative of its different functional properties. This information extent our understanding of the diversity of M cells and provides important basic knowledge for further research on the actual role of villous M cells in neonate.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400011en10.4067/S0717-95022015000400011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	M Cells Villous epithelium Small intestine Newborn piglets
spellingShingle	M Cells Villous epithelium Small intestine Newborn piglets Renfeng,Li Xiangqin,Tian Songlin,Qiao Yanyan,Yang Enmin,Zhou Gaiping,Zhang Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
description	Microfold (M) cells act as antigen-sampling sites for initiating antigen specific mucosal immune responses, but they may also provide a gateway for enteropathogen entry. In this study we demonstrated villous M cells by morphological and immunohistochemical methods to be present in the three regions of the small intestine from newborn piglets. Immunohistochemical analysis, using anti- cytokeratin 18 (CK18) primary antibodies, showed a gradually decreased density of M cells from the lower crypt epithelium to the upper villous epithelium. The proportion of villous M cells was greater in the ileum than in the duodenum or the mid-jejunum. Ultrastructural observation revealed that villous M cells were mainly columnar in shape in the duodenum and the mid-jejunum, and appeared as more pocket-like structure in the ileum. These villous M cells exhibited short and irregular microvilli, rich vesicles and reduced glycocalyx, but lacked the lymphocyte-containing basolateral invagination. Our results support evidence that M cells can develop in the small intestinal villous epithelium of newborn piglets, implying that villous M cells may begin developing in the pig's small intestine during fetal stages, which depends neither on the influence of the mucosal lymphoid tissue nor the antigen from the intestinal lumen stimulation. In addition, the variable morphology and heterogeneity distribution of villous M cells in the three regions of the small intestine may be indicative of its different functional properties. This information extent our understanding of the diversity of M cells and provides important basic knowledge for further research on the actual role of villous M cells in neonate.
author	Renfeng,Li Xiangqin,Tian Songlin,Qiao Yanyan,Yang Enmin,Zhou Gaiping,Zhang
author_facet	Renfeng,Li Xiangqin,Tian Songlin,Qiao Yanyan,Yang Enmin,Zhou Gaiping,Zhang
author_sort	Renfeng,Li
title	Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
title_short	Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
title_full	Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
title_fullStr	Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
title_full_unstemmed	Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets
title_sort	morphological and immunohistochemical identification of villous m cells in the small intestine of newborn piglets
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400011
work_keys_str_mv	AT renfengli morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets AT xiangqintian morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets AT songlinqiao morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets AT yanyanyang morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets AT enminzhou morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets AT gaipingzhang morphologicalandimmunohistochemicalidentificationofvillousmcellsinthesmallintestineofnewbornpiglets
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Morphological and Immunohistochemical Identification of Villous M Cells in the Small Intestine of Newborn Piglets

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