Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

The purpose of this study, ischemia reperfusion injury in rats, Potentilla fulgens is to investigate the protective effects. Wistar albino rats (n= 30) weighing 180-220 g were used in the experiment. Group 1 animals underwent sham laparotomy without ischemia-reperfusion injury. Group 2 animals under...

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Autores principales:	Tunç,Nurettin, Tas,Mahmut, Görük,Mücahit, Kirman,Günsel, Deveci,Engin
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Intestinal ischemia Proliferating Cell Nuclear Antigen (PCNA) Rat Tunnel method
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400020
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spelling	oai:scielo:S0717-950220150004000202016-03-22Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion InjuryTunç,NurettinTas,MahmutGörük,MücahitKirman,GünselDeveci,Engin Intestinal ischemia Proliferating Cell Nuclear Antigen (PCNA) Rat Tunnel method The purpose of this study, ischemia reperfusion injury in rats, Potentilla fulgens is to investigate the protective effects. Wistar albino rats (n= 30) weighing 180-220 g were used in the experiment. Group 1 animals underwent sham laparotomy without ischemia-reperfusion injury. Group 2 animals underwent laparotomy and occlusion of superior mesenteric arteries for 30 min followed by 20 min of reperfusion without pretreatment. The Potentilla fulgens group received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before Ischemia-reperfusion injury. There was a significant difference between the group with ischemia-reperfusion group Potentilla fulgens (p<0.0001). In statistical analysis of the MDA level, data were obtained after a respective measurement in all groups. Potentilla fulgens group with ischemia-reperfusion group was a significant decrease in MDA (p<0.001). In the period after ischemia-reperfusion, marked PCNA immunoreactivities were observed in the nuclei of crypt and villus cell. In ischemia reperfusion group, the number of PCNA immunoreactivity is quite advanced and they extended throughout the middle part of the intestine folds. The number of TUNEL-positive nuclei were also developed. In ischemia-reperfusion plus P. fulgens group, the intestinal epithelium with only a few PCNA immunoreactive nuclei. TUNEL positive nuclei were noted in the gut lumen and mucosal close differentiated goblet cells. We showed that Potentilla fulgens extract significantly prevented mucosal lesions caused by intestinal ischemia-reperfusion.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400020en10.4067/S0717-95022015000400020
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Intestinal ischemia Proliferating Cell Nuclear Antigen (PCNA) Rat Tunnel method
spellingShingle	Intestinal ischemia Proliferating Cell Nuclear Antigen (PCNA) Rat Tunnel method Tunç,Nurettin Tas,Mahmut Görük,Mücahit Kirman,Günsel Deveci,Engin Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
description	The purpose of this study, ischemia reperfusion injury in rats, Potentilla fulgens is to investigate the protective effects. Wistar albino rats (n= 30) weighing 180-220 g were used in the experiment. Group 1 animals underwent sham laparotomy without ischemia-reperfusion injury. Group 2 animals underwent laparotomy and occlusion of superior mesenteric arteries for 30 min followed by 20 min of reperfusion without pretreatment. The Potentilla fulgens group received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before Ischemia-reperfusion injury. There was a significant difference between the group with ischemia-reperfusion group Potentilla fulgens (p<0.0001). In statistical analysis of the MDA level, data were obtained after a respective measurement in all groups. Potentilla fulgens group with ischemia-reperfusion group was a significant decrease in MDA (p<0.001). In the period after ischemia-reperfusion, marked PCNA immunoreactivities were observed in the nuclei of crypt and villus cell. In ischemia reperfusion group, the number of PCNA immunoreactivity is quite advanced and they extended throughout the middle part of the intestine folds. The number of TUNEL-positive nuclei were also developed. In ischemia-reperfusion plus P. fulgens group, the intestinal epithelium with only a few PCNA immunoreactive nuclei. TUNEL positive nuclei were noted in the gut lumen and mucosal close differentiated goblet cells. We showed that Potentilla fulgens extract significantly prevented mucosal lesions caused by intestinal ischemia-reperfusion.
author	Tunç,Nurettin Tas,Mahmut Görük,Mücahit Kirman,Günsel Deveci,Engin
author_facet	Tunç,Nurettin Tas,Mahmut Görük,Mücahit Kirman,Günsel Deveci,Engin
author_sort	Tunç,Nurettin
title	Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
title_short	Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
title_full	Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
title_fullStr	Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
title_full_unstemmed	Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
title_sort	effects of potentilla fulgens as prophylactic agent in intestinal ischemia reperfusion injury
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400020
work_keys_str_mv	AT tuncnurettin effectsofpotentillafulgensasprophylacticagentinintestinalischemiareperfusioninjury AT tasmahmut effectsofpotentillafulgensasprophylacticagentinintestinalischemiareperfusioninjury AT gorukmucahit effectsofpotentillafulgensasprophylacticagentinintestinalischemiareperfusioninjury AT kirmangunsel effectsofpotentillafulgensasprophylacticagentinintestinalischemiareperfusioninjury AT deveciengin effectsofpotentillafulgensasprophylacticagentinintestinalischemiareperfusioninjury
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Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury

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