Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells

The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muhammed,Aisha A, Yunus,Mohd Heikal M, Saim,Aminuddin B, Idrus,Ruszymah B. H
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Human nasal respiratory epithelium Immunostaining Mesenchymal stem cells marker Tissue engineering Nasal turbinate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400047
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022015000400047
record_format	dspace
spelling	oai:scielo:S0717-950220150004000472016-03-22Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial CellsMuhammed,Aisha AYunus,Mohd Heikal MSaim,Aminuddin BIdrus,Ruszymah B. H Human nasal respiratory epithelium Immunostaining Mesenchymal stem cells marker Tissue engineering Nasal turbinate The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are responsible for some of these cells unique properties. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. Respiratory epithelial cells were cultured using co-culture technique, fibroblasts was removed at confluence leaving respiratory epithelial cells, which were passage further to passage 4. Cells were evaluated for mesenchymal stem cell markers that were CD73, CD90, CD105 and the hematopoietic stem cell marker CD45 at passage 1 (P1) and passage 4 (P4) using Flow cytometry and Immunocytochemistry techniques. Respiratory epithelial cells expressed the mesenchymal stem cell markers at P1 and maintain the expression these markers until P4. Using both techniques, to compare the values of mesenchymal stem cell markers expression at P1 to P4 there was no significant difference. This study indicates that respiratory epithelial cells derived from nasal turbinate retain some of mesenchymal stem cells properties even after serial passages. Both methods of Immunophenotyping are comparable.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400047en10.4067/S0717-95022015000400047
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Human nasal respiratory epithelium Immunostaining Mesenchymal stem cells marker Tissue engineering Nasal turbinate
spellingShingle	Human nasal respiratory epithelium Immunostaining Mesenchymal stem cells marker Tissue engineering Nasal turbinate Muhammed,Aisha A Yunus,Mohd Heikal M Saim,Aminuddin B Idrus,Ruszymah B. H Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
description	The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are responsible for some of these cells unique properties. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. Respiratory epithelial cells were cultured using co-culture technique, fibroblasts was removed at confluence leaving respiratory epithelial cells, which were passage further to passage 4. Cells were evaluated for mesenchymal stem cell markers that were CD73, CD90, CD105 and the hematopoietic stem cell marker CD45 at passage 1 (P1) and passage 4 (P4) using Flow cytometry and Immunocytochemistry techniques. Respiratory epithelial cells expressed the mesenchymal stem cell markers at P1 and maintain the expression these markers until P4. Using both techniques, to compare the values of mesenchymal stem cell markers expression at P1 to P4 there was no significant difference. This study indicates that respiratory epithelial cells derived from nasal turbinate retain some of mesenchymal stem cells properties even after serial passages. Both methods of Immunophenotyping are comparable.
author	Muhammed,Aisha A Yunus,Mohd Heikal M Saim,Aminuddin B Idrus,Ruszymah B. H
author_facet	Muhammed,Aisha A Yunus,Mohd Heikal M Saim,Aminuddin B Idrus,Ruszymah B. H
author_sort	Muhammed,Aisha A
title	Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
title_short	Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
title_full	Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
title_fullStr	Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
title_full_unstemmed	Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells
title_sort	expression of mesenchymal stem cell phenotype in human nasal respiratory epithelial cells
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400047
work_keys_str_mv	AT muhammedaishaa expressionofmesenchymalstemcellphenotypeinhumannasalrespiratoryepithelialcells AT yunusmohdheikalm expressionofmesenchymalstemcellphenotypeinhumannasalrespiratoryepithelialcells AT saimaminuddinb expressionofmesenchymalstemcellphenotypeinhumannasalrespiratoryepithelialcells AT idrusruszymahbh expressionofmesenchymalstemcellphenotypeinhumannasalrespiratoryepithelialcells
_version_	1718444941478199296

Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells

Ejemplares similares