Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats

This study investigated the effects of visfatin on the structure and the immunity levels in the small intestine of LPS-induced rats. Forty Wistar male and female SPF rats were randomly and equally divided into four groups: the saline (control), vistfatin, lipopolysaccharide (LPS), and visfatin+LPS c...

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Autores principales:	Zhou,Ying, Cui,Lu, Yuan,Huairui, Xiao,Ke, Khan,Faheem Ahmed, Guo,Liang, Yang,Zhi, Song,Hui
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2015
Materias:	Visfatin Intestinal mucosa LPSinduced rats IgA Inflammatory factor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400051
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spelling	oai:scielo:S0717-950220150004000512016-03-22Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced RatsZhou,YingCui,LuYuan,HuairuiXiao,KeKhan,Faheem AhmedGuo,LiangYang,ZhiSong,Hui Visfatin Intestinal mucosa LPSinduced rats IgA Inflammatory factor This study investigated the effects of visfatin on the structure and the immunity levels in the small intestine of LPS-induced rats. Forty Wistar male and female SPF rats were randomly and equally divided into four groups: the saline (control), vistfatin, lipopolysaccharide (LPS), and visfatin+LPS co-stimulated. The functions of visfatin in the intestinal mucosal immunity were investigated by examining the variation of tissue structure, inflammation and immunity-related proteins in the intestine of immunologically stressed rats using HE staining, ELISA, immunohistochemistry and Western Blot. The results showed that, when compared with the control group, the visfatin-treated group showed a decrease in the intestinal villus height and width, and a significant increase in the levels of IL-6 and TNF-ð as well as Immunoglobulin A (IgA) positive cells. Additionally, when compared with the LPS-treated group, the visfatin+LPS co-stimulated group showed a decrease in the villus height and width as well as the levels of IL-6 and TNF-ð, and an increase in IgA levels, implying a shrinking response to LPS injection. All the results suggest that, under normal physiological conditions, visfatin disturbs the body's homeostasis and causes intestinal villus atrophy by increasing IgA expression. While under immune response conditions, LPS acts as an exogenous antigen to promote visfatin against LPS-induced inflammation by decreasing the expression of IgA. Under immune stress conditions, visfatin as an exogenous stimulus promotes the immune response by regulating the protein levels of IL-6, TNF-ð and IgA.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.33 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400051en10.4067/S0717-95022015000400051
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Visfatin Intestinal mucosa LPSinduced rats IgA Inflammatory factor
spellingShingle	Visfatin Intestinal mucosa LPSinduced rats IgA Inflammatory factor Zhou,Ying Cui,Lu Yuan,Huairui Xiao,Ke Khan,Faheem Ahmed Guo,Liang Yang,Zhi Song,Hui Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
description	This study investigated the effects of visfatin on the structure and the immunity levels in the small intestine of LPS-induced rats. Forty Wistar male and female SPF rats were randomly and equally divided into four groups: the saline (control), vistfatin, lipopolysaccharide (LPS), and visfatin+LPS co-stimulated. The functions of visfatin in the intestinal mucosal immunity were investigated by examining the variation of tissue structure, inflammation and immunity-related proteins in the intestine of immunologically stressed rats using HE staining, ELISA, immunohistochemistry and Western Blot. The results showed that, when compared with the control group, the visfatin-treated group showed a decrease in the intestinal villus height and width, and a significant increase in the levels of IL-6 and TNF-ð as well as Immunoglobulin A (IgA) positive cells. Additionally, when compared with the LPS-treated group, the visfatin+LPS co-stimulated group showed a decrease in the villus height and width as well as the levels of IL-6 and TNF-ð, and an increase in IgA levels, implying a shrinking response to LPS injection. All the results suggest that, under normal physiological conditions, visfatin disturbs the body's homeostasis and causes intestinal villus atrophy by increasing IgA expression. While under immune response conditions, LPS acts as an exogenous antigen to promote visfatin against LPS-induced inflammation by decreasing the expression of IgA. Under immune stress conditions, visfatin as an exogenous stimulus promotes the immune response by regulating the protein levels of IL-6, TNF-ð and IgA.
author	Zhou,Ying Cui,Lu Yuan,Huairui Xiao,Ke Khan,Faheem Ahmed Guo,Liang Yang,Zhi Song,Hui
author_facet	Zhou,Ying Cui,Lu Yuan,Huairui Xiao,Ke Khan,Faheem Ahmed Guo,Liang Yang,Zhi Song,Hui
author_sort	Zhou,Ying
title	Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
title_short	Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
title_full	Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
title_fullStr	Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
title_full_unstemmed	Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats
title_sort	effect of visfatin on the structure and immune levels in the small intestine of lps-induced rats
publisher	Sociedad Chilena de Anatomía
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400051
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Effect of Visfatin on the Structure and Immune Levels in the Small Intestine of LPS-Induced Rats

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