Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions

Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of...

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Autor principal: Rosero-Salazar,Doris Haydee
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2016
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019
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spelling oai:scielo:S0717-950220160001000192016-05-02Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on RegionsRosero-Salazar,Doris Haydee Histochemistry Ischemia Reperfusion Image analysis Segmentation Histoquímica Isquemia Reperfusión Análisis de imágenes Segmentación Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using practical procedures of image analysis in the diagnosis of tisular abnormalities.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019en10.4067/S0717-95022016000100019
institution Scielo Chile
collection Scielo Chile
language English
topic Histochemistry
Ischemia
Reperfusion
Image analysis
Segmentation
Histoquímica
Isquemia
Reperfusión
Análisis de imágenes
Segmentación
spellingShingle Histochemistry
Ischemia
Reperfusion
Image analysis
Segmentation
Histoquímica
Isquemia
Reperfusión
Análisis de imágenes
Segmentación
Rosero-Salazar,Doris Haydee
Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
description Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using practical procedures of image analysis in the diagnosis of tisular abnormalities.
author Rosero-Salazar,Doris Haydee
author_facet Rosero-Salazar,Doris Haydee
author_sort Rosero-Salazar,Doris Haydee
title Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
title_short Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
title_full Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
title_fullStr Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
title_full_unstemmed Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions
title_sort image analysis of oxidative and glycolytic muscle fibers during reperfusion injury by segmentation based on regions
publisher Sociedad Chilena de Anatomía
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019
work_keys_str_mv AT roserosalazardorishaydee imageanalysisofoxidativeandglycolyticmusclefibersduringreperfusioninjurybysegmentationbasedonregions
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