Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes

Gestational diabetes mellitus (GDM) defined as impaired glucose tolerance affects approximately 6 % of all pregnant women who have never before had diabetes, but who do have high blood glucose levels during pregnancy. This study was done to evaluate the apoptosis in the neuronal cells in the CA1, CA...

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Autores principales:	Ghafari,Soraya, Asadi,Ebrahim, Shabani,Ronak, Golalipour,Mohammad Jafar
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Gestational diabetes Hippocampus Neuron Apoptosis TUNEL Assay Dentate gyrus Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100029
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spelling	oai:scielo:S0717-950220160001000292016-05-02Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational DiabetesGhafari,SorayaAsadi,EbrahimShabani,RonakGolalipour,Mohammad Jafar Gestational diabetes Hippocampus Neuron Apoptosis TUNEL Assay Dentate gyrus Rat Gestational diabetes mellitus (GDM) defined as impaired glucose tolerance affects approximately 6 % of all pregnant women who have never before had diabetes, but who do have high blood glucose levels during pregnancy. This study was done to evaluate the apoptosis in the neuronal cells in the CA1, CA2 and CA3 subfields of hippocampus and dentate gyrus in offspring of gestational diabetes at the 7, 21 and 28 d in postnatal rats. Thirty Wistar rat dams were randomly allocated in control and diabetic group. Dams in diabetic group were received 40 mg/kg/BW of streptozotocin at the first day of gestation and control groups received an equivalent volume normal saline injection intraperitoneally (IP). Six offspring of GDM and control dams, at the 7, 21, 28 postnatal day were randomly were sacrificed quickly with anesthesia. The coronal sections of brain serially collected. The apoptosis neurons were evaluated with TUNEL Assay. In the CA1, the number of apoptotic cells in 7, 21 and 28 d of postnatal life were significantly increased in GDM compared to controls (P<0.001). In the CA2, CA3 the number of apoptotic cells in 7, 21 and 28 d age-old offspring were significantly increased in GDM compared to controls (P<0.001). In the dentate gyrus, the number of apoptotic cells in 7, 21 and 28 d of postnatal life were significantly increased in GDM compared to controls (P<0.01). This study showed that the uncontrolled gestational diabetes significantly increases neuronal apoptosis in hippocampal and dentate gyrus in rat offspring.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100029en10.4067/S0717-95022016000100029
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gestational diabetes Hippocampus Neuron Apoptosis TUNEL Assay Dentate gyrus Rat
spellingShingle	Gestational diabetes Hippocampus Neuron Apoptosis TUNEL Assay Dentate gyrus Rat Ghafari,Soraya Asadi,Ebrahim Shabani,Ronak Golalipour,Mohammad Jafar Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
description	Gestational diabetes mellitus (GDM) defined as impaired glucose tolerance affects approximately 6 % of all pregnant women who have never before had diabetes, but who do have high blood glucose levels during pregnancy. This study was done to evaluate the apoptosis in the neuronal cells in the CA1, CA2 and CA3 subfields of hippocampus and dentate gyrus in offspring of gestational diabetes at the 7, 21 and 28 d in postnatal rats. Thirty Wistar rat dams were randomly allocated in control and diabetic group. Dams in diabetic group were received 40 mg/kg/BW of streptozotocin at the first day of gestation and control groups received an equivalent volume normal saline injection intraperitoneally (IP). Six offspring of GDM and control dams, at the 7, 21, 28 postnatal day were randomly were sacrificed quickly with anesthesia. The coronal sections of brain serially collected. The apoptosis neurons were evaluated with TUNEL Assay. In the CA1, the number of apoptotic cells in 7, 21 and 28 d of postnatal life were significantly increased in GDM compared to controls (P<0.001). In the CA2, CA3 the number of apoptotic cells in 7, 21 and 28 d age-old offspring were significantly increased in GDM compared to controls (P<0.001). In the dentate gyrus, the number of apoptotic cells in 7, 21 and 28 d of postnatal life were significantly increased in GDM compared to controls (P<0.01). This study showed that the uncontrolled gestational diabetes significantly increases neuronal apoptosis in hippocampal and dentate gyrus in rat offspring.
author	Ghafari,Soraya Asadi,Ebrahim Shabani,Ronak Golalipour,Mohammad Jafar
author_facet	Ghafari,Soraya Asadi,Ebrahim Shabani,Ronak Golalipour,Mohammad Jafar
author_sort	Ghafari,Soraya
title	Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
title_short	Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
title_full	Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
title_fullStr	Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
title_full_unstemmed	Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes
title_sort	hippocampal neuronal apoptosis in rat offspring due to gestational diabetes
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100029
work_keys_str_mv	AT ghafarisoraya hippocampalneuronalapoptosisinratoffspringduetogestationaldiabetes AT asadiebrahim hippocampalneuronalapoptosisinratoffspringduetogestationaldiabetes AT shabanironak hippocampalneuronalapoptosisinratoffspringduetogestationaldiabetes AT golalipourmohammadjafar hippocampalneuronalapoptosisinratoffspringduetogestationaldiabetes
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Hippocampal Neuronal Apoptosis in Rat Offspring Due to Gestational Diabetes

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