Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction

Protein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the...

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Autores principales:	Jeronimo,L. C, Confortim,H. D, Centenaro,L. A, Brancalhão,R. M. C, Pinheiro,P. F. F, Matheus,S. M. M, Torrejais,M. M
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Morphology Morphometry Protein restriction Extensor digitorum longus muscle Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100057
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spelling	oai:scielo:S0717-950220160001000572016-05-02Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein RestrictionJeronimo,L. CConfortim,H. DCentenaro,L. ABrancalhão,R. M. CPinheiro,P. F. FMatheus,S. M. MTorrejais,M. M Morphology Morphometry Protein restriction Extensor digitorum longus muscle Rat Protein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the extensor digitorum longus (EDL) muscle in rats at 365 days of age, submitted to maternal protein restriction during the gestation and lactation periods. Wistar rats were divided into two groups: a Control Group - mothers fed a normal-protein diet (17 % protein) during pregnancy and lactation; and a Restricted Group - mothers fed a low-protein diet (6 % protein) during pregnancy and lactation. The pups were kept with the mother throughout the lactation period (21 days), after which the offspring received a normal protein diet until 365 days of age. Histological (HE) and histoenzymological (NADH-TR) studies were conducted on the muscle fibers. The muscle was subjected to Nonspecific Esterase reaction to stain the Neuromuscular Junctions. Regarding the animals from the restricted group: the histologic analysis of the muscle fibers showed the presence of centralized nuclei and a diminished area; the histoenzymological study showed the different types of muscle fibers were randomly distributed in the EDL muscle and the area of the Type IIa muscle fiber was smaller; the ultrastructural study revealed disorganization of the Z line, and the presence of lipid droplets and vacuoles containing myelin figures in subsarcolemmal and intramiofibrilar regions; while the analysis of the NMJs exhibited no significant differences between the groups. Protein restriction in the pregnancy and lactation period may have affected the development of skeletal muscle, producing a permanent muscle-fiber deficit in the EDL muscle of the offspring.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100057en10.4067/S0717-95022016000100057
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Morphology Morphometry Protein restriction Extensor digitorum longus muscle Rat
spellingShingle	Morphology Morphometry Protein restriction Extensor digitorum longus muscle Rat Jeronimo,L. C Confortim,H. D Centenaro,L. A Brancalhão,R. M. C Pinheiro,P. F. F Matheus,S. M. M Torrejais,M. M Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
description	Protein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the extensor digitorum longus (EDL) muscle in rats at 365 days of age, submitted to maternal protein restriction during the gestation and lactation periods. Wistar rats were divided into two groups: a Control Group - mothers fed a normal-protein diet (17 % protein) during pregnancy and lactation; and a Restricted Group - mothers fed a low-protein diet (6 % protein) during pregnancy and lactation. The pups were kept with the mother throughout the lactation period (21 days), after which the offspring received a normal protein diet until 365 days of age. Histological (HE) and histoenzymological (NADH-TR) studies were conducted on the muscle fibers. The muscle was subjected to Nonspecific Esterase reaction to stain the Neuromuscular Junctions. Regarding the animals from the restricted group: the histologic analysis of the muscle fibers showed the presence of centralized nuclei and a diminished area; the histoenzymological study showed the different types of muscle fibers were randomly distributed in the EDL muscle and the area of the Type IIa muscle fiber was smaller; the ultrastructural study revealed disorganization of the Z line, and the presence of lipid droplets and vacuoles containing myelin figures in subsarcolemmal and intramiofibrilar regions; while the analysis of the NMJs exhibited no significant differences between the groups. Protein restriction in the pregnancy and lactation period may have affected the development of skeletal muscle, producing a permanent muscle-fiber deficit in the EDL muscle of the offspring.
author	Jeronimo,L. C Confortim,H. D Centenaro,L. A Brancalhão,R. M. C Pinheiro,P. F. F Matheus,S. M. M Torrejais,M. M
author_facet	Jeronimo,L. C Confortim,H. D Centenaro,L. A Brancalhão,R. M. C Pinheiro,P. F. F Matheus,S. M. M Torrejais,M. M
author_sort	Jeronimo,L. C
title	Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
title_short	Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
title_full	Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
title_fullStr	Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
title_full_unstemmed	Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction
title_sort	morphological and morphometric study of the muscle fibers and neuromuscular junctions of the extensor digitorum longus in aged rats submitted to maternal protein restriction
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100057
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Morphological and Morphometric Study of the Muscle Fibers and Neuromuscular Junctions of the Extensor Digitorum Longus in Aged Rats Submitted to Maternal Protein Restriction

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