Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ
By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proxim...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2016
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oai:scielo:S0717-950220160002000102016-07-13Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol KinaseζHipkaeo,WiphawiTechataweewan,NawapornSakaew,WarapornMaleewong,WanchaiSripa,BanchobChomphoo,SurangHozumi,YasukazuGoto,KaoruKondo,Hisatake DGK<img border=0 width=17 height=33 src="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg" alt="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg"> Immunoreactivity Peripheral neurons Glia Opisthorchis viverrini Ultrastructure By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the cells. Thin cellular processes without the immunoreactivity were found to enclose thinly the entire surfaces of the immunostained cells and processes, and they were in continuity with the interstitial partition-like processes which contained nuclei and aggregation of microfibrils at some distance from the cytoplasmic envelopes. The present finding suggests the possibility that the immunostained cells were peripheral neurons enveloped by peripheral glia and that the glia are of mesenchymal origin because of their cytoplasmic continuity to the interstitial partition-like processes. The motor or sensory nature of the neurons remains to be elucidated.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200010en10.4067/S0717-95022016000200010 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
DGK<img border=0 width=17 height=33 src="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg" alt="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg"> Immunoreactivity Peripheral neurons Glia Opisthorchis viverrini Ultrastructure |
| spellingShingle |
DGK<img border=0 width=17 height=33 src="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg" alt="http://fbpe/img/ijmorphol/v34n2/art10_simbolo1.jpg"> Immunoreactivity Peripheral neurons Glia Opisthorchis viverrini Ultrastructure Hipkaeo,Wiphawi Techataweewan,Nawaporn Sakaew,Waraporn Maleewong,Wanchai Sripa,Banchob Chomphoo,Surang Hozumi,Yasukazu Goto,Kaoru Kondo,Hisatake Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| description |
By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the cells. Thin cellular processes without the immunoreactivity were found to enclose thinly the entire surfaces of the immunostained cells and processes, and they were in continuity with the interstitial partition-like processes which contained nuclei and aggregation of microfibrils at some distance from the cytoplasmic envelopes. The present finding suggests the possibility that the immunostained cells were peripheral neurons enveloped by peripheral glia and that the glia are of mesenchymal origin because of their cytoplasmic continuity to the interstitial partition-like processes. The motor or sensory nature of the neurons remains to be elucidated. |
| author |
Hipkaeo,Wiphawi Techataweewan,Nawaporn Sakaew,Waraporn Maleewong,Wanchai Sripa,Banchob Chomphoo,Surang Hozumi,Yasukazu Goto,Kaoru Kondo,Hisatake |
| author_facet |
Hipkaeo,Wiphawi Techataweewan,Nawaporn Sakaew,Waraporn Maleewong,Wanchai Sripa,Banchob Chomphoo,Surang Hozumi,Yasukazu Goto,Kaoru Kondo,Hisatake |
| author_sort |
Hipkaeo,Wiphawi |
| title |
Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| title_short |
Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| title_full |
Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| title_fullStr |
Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| title_full_unstemmed |
Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ |
| title_sort |
identification in immuno-electron microscopy of solitary multi-polar peripheral neurons of adult opisthorchis viverrini by antibody against rat diacylglycerol kinaseζ |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2016 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200010 |
| work_keys_str_mv |
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| _version_ |
1718444957529800704 |