Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

In birds, male embryo the gonads develop bilateral testes, in which both left and right sides produce functional spermatozoa, whereas female embryo, only the left gonad develops into a functional ovary. Estrogen plays a key role in avian sex determination in both sexes by binding to the estrogen rec...

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Autores principales:	Intarapat,Sittipon, Sailasuta,Achariya, Satayalai,Orawan
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Estrogen receptor Embryonic gonad Gonadal differentiation Quail embryo
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200026
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spelling	oai:scielo:S0717-950220160002000262016-07-13Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal DifferentiationIntarapat,SittiponSailasuta,AchariyaSatayalai,Orawan Estrogen receptor Embryonic gonad Gonadal differentiation Quail embryo In birds, male embryo the gonads develop bilateral testes, in which both left and right sides produce functional spermatozoa, whereas female embryo, only the left gonad develops into a functional ovary. Estrogen plays a key role in avian sex determination in both sexes by binding to the estrogen receptor (ER). Surprisingly, chicken estrogen receptor (cER) mRNA is expressed in both sexes; moreover; its expression is only expressed in the left male gonad. The present study aimed to localize ER protein in the left gonad of male quail embryo using immunohistochemistry. The 8-day-old male quail embryos whose embryonic sex distinguished by gonadal morphology were studied. Histology of the left male gonad displayed thin cortex containing 1 to 2 layers of the germinal epithelium, while testicular cords were observed in the medulla. ER-immunoreactive cells were only found in the germinal epithelium but not in the medulla. Localization of ER was detected in the nucleus and cytoplasm of the germinal epithelial cells. The number of ER-immunoreactive cells counted in upper, lateral, and lower regions of the germinal epithelium was 18.20±1.892, 17.60±1.887, and 16.20±1.290, respectively. This study shows the first evidence for expression of ER protein in the left male gonad of the avian embryo, indicating that ER plays a role in avian gonadal sex differentiation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200026en10.4067/S0717-95022016000200026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Estrogen receptor Embryonic gonad Gonadal differentiation Quail embryo
spellingShingle	Estrogen receptor Embryonic gonad Gonadal differentiation Quail embryo Intarapat,Sittipon Sailasuta,Achariya Satayalai,Orawan Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
description	In birds, male embryo the gonads develop bilateral testes, in which both left and right sides produce functional spermatozoa, whereas female embryo, only the left gonad develops into a functional ovary. Estrogen plays a key role in avian sex determination in both sexes by binding to the estrogen receptor (ER). Surprisingly, chicken estrogen receptor (cER) mRNA is expressed in both sexes; moreover; its expression is only expressed in the left male gonad. The present study aimed to localize ER protein in the left gonad of male quail embryo using immunohistochemistry. The 8-day-old male quail embryos whose embryonic sex distinguished by gonadal morphology were studied. Histology of the left male gonad displayed thin cortex containing 1 to 2 layers of the germinal epithelium, while testicular cords were observed in the medulla. ER-immunoreactive cells were only found in the germinal epithelium but not in the medulla. Localization of ER was detected in the nucleus and cytoplasm of the germinal epithelial cells. The number of ER-immunoreactive cells counted in upper, lateral, and lower regions of the germinal epithelium was 18.20±1.892, 17.60±1.887, and 16.20±1.290, respectively. This study shows the first evidence for expression of ER protein in the left male gonad of the avian embryo, indicating that ER plays a role in avian gonadal sex differentiation.
author	Intarapat,Sittipon Sailasuta,Achariya Satayalai,Orawan
author_facet	Intarapat,Sittipon Sailasuta,Achariya Satayalai,Orawan
author_sort	Intarapat,Sittipon
title	Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
title_short	Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
title_full	Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
title_fullStr	Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
title_full_unstemmed	Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation
title_sort	immunohistochemical localization of estrogen receptor in the embryonic gonad of male quail embryo during gonadal differentiation
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200026
work_keys_str_mv	AT intarapatsittipon immunohistochemicallocalizationofestrogenreceptorintheembryonicgonadofmalequailembryoduringgonadaldifferentiation AT sailasutaachariya immunohistochemicallocalizationofestrogenreceptorintheembryonicgonadofmalequailembryoduringgonadaldifferentiation AT satayalaiorawan immunohistochemicallocalizationofestrogenreceptorintheembryonicgonadofmalequailembryoduringgonadaldifferentiation
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Immunohistochemical Localization of Estrogen Receptor in the Embryonic Gonad of Male Quail Embryo During Gonadal Differentiation

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