MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

MatrigelBD is a hydrogel scaffold with three-dimensional intercrossed networks of hydrophilic polymers with high water content. Human gingival tissue might represent a better source of MSCs, allowing these cells to be easily obtained in a relatively non-invasive way. The objective of this study was...

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Autores principales:	Brizuela,Claudia, SaintJean,Nicole, Garchitorena,Nicolas, Rodriguez,Ismael, Inostroza,Carolina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Adult stem cell research Hydrogels Tissue scaffolds Regeneration Materials testing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200043
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spelling	oai:scielo:S0717-950220160002000432016-07-13MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells ProliferationBrizuela,ClaudiaSaintJean,NicoleGarchitorena,NicolasRodriguez,IsmaelInostroza,Carolina Adult stem cell research Hydrogels Tissue scaffolds Regeneration Materials testing MatrigelBD is a hydrogel scaffold with three-dimensional intercrossed networks of hydrophilic polymers with high water content. Human gingival tissue might represent a better source of MSCs, allowing these cells to be easily obtained in a relatively non-invasive way. The objective of this study was to evaluate the biocompatibility of MatrigelBD with GMSCs in vitro. Gingival connective tissue samples were obtained from healthy donors. Fresh tissue was minced and cultured during two weeks, after which cells at passage fourth were analyzed for their immune phenotype by flow cytometry. Differentiation into osteogenic, chondrogenic, and adipogenic lineages was induced and evaluated by culture staining. The "construct" was made of MatrigelBD with GMSC. To assess the biocompatibility, an MTT cellular proliferation assay was performed. The differentiation potential of the cells toward the osteogenic, adipogenic, and chondrogenic lineages was analyzed after 21 days of growth in MatrigelBD with induction differentiation media. The MTT analysis showed that MatrigelBD stimulated cell proliferation; the GMSCs maintained the expression of MSC markers. Importantly, the growth of GMSCs within the MatrigelBD did not interfere with the cell differentiation potential. These findings indicate that MatrigelBD is biocompatible with GMSCs, and this matrix improves cell proliferation in vitro.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200043en10.4067/S0717-95022016000200043
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adult stem cell research Hydrogels Tissue scaffolds Regeneration Materials testing
spellingShingle	Adult stem cell research Hydrogels Tissue scaffolds Regeneration Materials testing Brizuela,Claudia SaintJean,Nicole Garchitorena,Nicolas Rodriguez,Ismael Inostroza,Carolina MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
description	MatrigelBD is a hydrogel scaffold with three-dimensional intercrossed networks of hydrophilic polymers with high water content. Human gingival tissue might represent a better source of MSCs, allowing these cells to be easily obtained in a relatively non-invasive way. The objective of this study was to evaluate the biocompatibility of MatrigelBD with GMSCs in vitro. Gingival connective tissue samples were obtained from healthy donors. Fresh tissue was minced and cultured during two weeks, after which cells at passage fourth were analyzed for their immune phenotype by flow cytometry. Differentiation into osteogenic, chondrogenic, and adipogenic lineages was induced and evaluated by culture staining. The "construct" was made of MatrigelBD with GMSC. To assess the biocompatibility, an MTT cellular proliferation assay was performed. The differentiation potential of the cells toward the osteogenic, adipogenic, and chondrogenic lineages was analyzed after 21 days of growth in MatrigelBD with induction differentiation media. The MTT analysis showed that MatrigelBD stimulated cell proliferation; the GMSCs maintained the expression of MSC markers. Importantly, the growth of GMSCs within the MatrigelBD did not interfere with the cell differentiation potential. These findings indicate that MatrigelBD is biocompatible with GMSCs, and this matrix improves cell proliferation in vitro.
author	Brizuela,Claudia SaintJean,Nicole Garchitorena,Nicolas Rodriguez,Ismael Inostroza,Carolina
author_facet	Brizuela,Claudia SaintJean,Nicole Garchitorena,Nicolas Rodriguez,Ismael Inostroza,Carolina
author_sort	Brizuela,Claudia
title	MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
title_short	MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
title_full	MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
title_fullStr	MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
title_full_unstemmed	MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation
title_sort	matrigelbd, a biocompatible scaffold that improves gingival mesenchymal stem cells proliferation
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200043
work_keys_str_mv	AT brizuelaclaudia matrigelbdabiocompatiblescaffoldthatimprovesgingivalmesenchymalstemcellsproliferation AT saintjeannicole matrigelbdabiocompatiblescaffoldthatimprovesgingivalmesenchymalstemcellsproliferation AT garchitorenanicolas matrigelbdabiocompatiblescaffoldthatimprovesgingivalmesenchymalstemcellsproliferation AT rodriguezismael matrigelbdabiocompatiblescaffoldthatimprovesgingivalmesenchymalstemcellsproliferation AT inostrozacarolina matrigelbdabiocompatiblescaffoldthatimprovesgingivalmesenchymalstemcellsproliferation
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MatrigelBD, A Biocompatible Scaffold that Improves Gingival Mesenchymal Stem Cells Proliferation

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