Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)

With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spa...

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Autores principales:	Rojas,Mariana, Saint-Pierre,Gustavo, Hartley,Ricardo, Vásquez,Bélgica, Conei,Daniel, del Sol,Mariano
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Sonic hedgehog Salmon fry Immunohistochemistry Antibody antiShh
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200054
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spelling	oai:scielo:S0717-950220160002000542016-07-13Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)Rojas,MarianaSaint-Pierre,GustavoHartley,RicardoVásquez,BélgicaConei,Danieldel Sol,Mariano Sonic hedgehog Salmon fry Immunohistochemistry Antibody antiShh With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The development was done with DAB (Vector) for 1-5 min. The negative control was incubated without primary antibody. As an internal positive control the notochord was considered. Serial sagittal sections were analyzed consigning tissues and organs marked positively and were described morphologically. The objective of recognizing the spatial and temporal location of Shh was achieved. The notochord, spinal cord neurons and ganglia, the basal layer of the skin and also the lepidotriquias escleroblastos were positively identified for Shh. Finally positivity was also observed in the intestine and renal tubules. The heterogeneity observed in the location of the Shh morphogen suggests its potential use as a marker of regulatory centers in Salmo salar, and a potential advantage in the diagnosis of malformations of salmon fry stage, in addition to a better understanding of tissue regeneration.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200054en10.4067/S0717-95022016000200054
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sonic hedgehog Salmon fry Immunohistochemistry Antibody antiShh
spellingShingle	Sonic hedgehog Salmon fry Immunohistochemistry Antibody antiShh Rojas,Mariana Saint-Pierre,Gustavo Hartley,Ricardo Vásquez,Bélgica Conei,Daniel del Sol,Mariano Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
description	With the purpose of carrying out a diagnosis of the different pathologies that affect the salmon fry stage (Salmo salar) and analyze the regeneration phases of the organizational centers and subjacent tissue in case of an amputation, we realized a study that allowed identifying the temporary and spatial location of the Sonic Hedgehog (Shh) morphogen in hatched fry stage. Fifteen salmon fry (Salmo salar) were used. They were anesthetized with 5 % benzocaine (BZ-20®, Veterquímica), fixed in 10 % buffered formalin, and embedded in paraffin, Shh polyclonal antibody (Santa Cruz H-160, rabbit) was used diluted at 1/100. They were subsequently rinsed in PBS-1 % Triton and incubated with anti-rabbit conjugated polymer antibody and HRP for 10-15 min. The development was done with DAB (Vector) for 1-5 min. The negative control was incubated without primary antibody. As an internal positive control the notochord was considered. Serial sagittal sections were analyzed consigning tissues and organs marked positively and were described morphologically. The objective of recognizing the spatial and temporal location of Shh was achieved. The notochord, spinal cord neurons and ganglia, the basal layer of the skin and also the lepidotriquias escleroblastos were positively identified for Shh. Finally positivity was also observed in the intestine and renal tubules. The heterogeneity observed in the location of the Shh morphogen suggests its potential use as a marker of regulatory centers in Salmo salar, and a potential advantage in the diagnosis of malformations of salmon fry stage, in addition to a better understanding of tissue regeneration.
author	Rojas,Mariana Saint-Pierre,Gustavo Hartley,Ricardo Vásquez,Bélgica Conei,Daniel del Sol,Mariano
author_facet	Rojas,Mariana Saint-Pierre,Gustavo Hartley,Ricardo Vásquez,Bélgica Conei,Daniel del Sol,Mariano
author_sort	Rojas,Mariana
title	Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
title_short	Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
title_full	Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
title_fullStr	Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
title_full_unstemmed	Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)
title_sort	immunolocalization of morphogen sonic hedgehog in salmon fry (salmo salar)
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200054
work_keys_str_mv	AT rojasmariana immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar AT saintpierregustavo immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar AT hartleyricardo immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar AT vasquezbelgica immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar AT coneidaniel immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar AT delsolmariano immunolocalizationofmorphogensonichedgehoginsalmonfrysalmosalar
_version_	1718444965765316608

Immunolocalization of Morphogen Sonic Hedgehog in Salmon Fry (Salmo salar)

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