Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks

Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on prol...

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Autores principales:	Sun,PengPeng, Luo,You, Wu,Xin Tong, Ansari,Abdur Rahman, Wang,Jing, Yang,KeLi, Xiao,Ke, Peng,KeMei
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Apoptosis Boron Intestine Ostrich chicks Proliferation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002
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spelling	oai:scielo:S0717-950220160003000022016-11-17Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich ChicksSun,PengPengLuo,YouWu,Xin TongAnsari,Abdur RahmanWang,JingYang,KeLiXiao,KePeng,KeMei Apoptosis Boron Intestine Ostrich chicks Proliferation Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end labeling (TUNEL) reaction and evaluated by integral optical density (IOD). Results showed that proliferationof intestinal cells significantly increased in groups of 80, 160, 320 and 640 mg/L. TUNEL reaction showed that apoptosis significantly decreased in 80 mg/L groups, while significantly increased in high dose of boron groups (320 and 640 mg/L), especially inepithelium. In conclusion, low dose of boron-supplemented water could promote cell proliferation and depress apoptosis, while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory adaption. These findings may support optimal dosage of boron that could protect the development of ostrich intestine, while high dosage of boron could suppress it, or even has toxic effects on it.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002en10.4067/S0717-95022016000300002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Apoptosis Boron Intestine Ostrich chicks Proliferation
spellingShingle	Apoptosis Boron Intestine Ostrich chicks Proliferation Sun,PengPeng Luo,You Wu,Xin Tong Ansari,Abdur Rahman Wang,Jing Yang,KeLi Xiao,Ke Peng,KeMei Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
description	Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end labeling (TUNEL) reaction and evaluated by integral optical density (IOD). Results showed that proliferationof intestinal cells significantly increased in groups of 80, 160, 320 and 640 mg/L. TUNEL reaction showed that apoptosis significantly decreased in 80 mg/L groups, while significantly increased in high dose of boron groups (320 and 640 mg/L), especially inepithelium. In conclusion, low dose of boron-supplemented water could promote cell proliferation and depress apoptosis, while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory adaption. These findings may support optimal dosage of boron that could protect the development of ostrich intestine, while high dosage of boron could suppress it, or even has toxic effects on it.
author	Sun,PengPeng Luo,You Wu,Xin Tong Ansari,Abdur Rahman Wang,Jing Yang,KeLi Xiao,Ke Peng,KeMei
author_facet	Sun,PengPeng Luo,You Wu,Xin Tong Ansari,Abdur Rahman Wang,Jing Yang,KeLi Xiao,Ke Peng,KeMei
author_sort	Sun,PengPeng
title	Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
title_short	Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
title_full	Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
title_fullStr	Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
title_full_unstemmed	Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
title_sort	effects of supplemental boron on intestinal proliferation and apoptosis in african ostrich chicks
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002
work_keys_str_mv	AT sunpengpeng effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT luoyou effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT wuxintong effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT ansariabdurrahman effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT wangjing effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT yangkeli effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT xiaoke effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks AT pengkemei effectsofsupplementalborononintestinalproliferationandapoptosisinafricanostrichchicks
_version_	1718444967086522368

Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks

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