Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice

Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal n...

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Autores principales:	Rengifo,Aura Caterine, Umbarila,Vanessa Jazmin, Garzón,Mary Janeth, Torres-Fernández,Orlando
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Rabies NeuN Cerebral cortex Mouse Immunohistochemistry Neurons
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031
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spelling	oai:scielo:S0717-950220160004000312017-02-01Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of MiceRengifo,Aura CaterineUmbarila,Vanessa JazminGarzón,Mary JanethTorres-Fernández,Orlando Rabies NeuN Cerebral cortex Mouse Immunohistochemistry Neurons Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. Rabies-infected mice were sacrificed at the terminal stage of the disease. Control mice were also euthanized at the same age. The brains were removed and cut into coronal sections on a vibratome. Immunohistochemistry was used to study the expression of NeuN in the motor area of the cerebral cortex. Neuronal counts, cellular optical densitometry and neuronal diameter measurements were performed to analyze the immunoreactivity of the protein. All parameters revealed decreased immunoreactivity for NeuN in cortical neurons of mice intracerebrally infected with rabies. In contrast, the changes were not statistically significant in mice inoculated intramuscularly. Either the immunoreactivity of NeuN or its expression is affected by the presence of rabies virus in the cerebral cortex depending on the inoculation route. These results contribute to the knowledge of the dynamics of cellular infection on rabies pathogenesis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031en10.4067/S0717-95022016000400031
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rabies NeuN Cerebral cortex Mouse Immunohistochemistry Neurons
spellingShingle	Rabies NeuN Cerebral cortex Mouse Immunohistochemistry Neurons Rengifo,Aura Caterine Umbarila,Vanessa Jazmin Garzón,Mary Janeth Torres-Fernández,Orlando Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
description	Rabies is a lethal disease caused by a neurotropic virus that produces inconspicuous morphological changes hardly observable with conventional histopathology. The fatal outcome caused by rabies could be attributed to specific biochemical changes that severely impact neuronal function. The neuronal nuclear protein (NeuN) has become a widely used neuronal marker for the research and the histopathological diagnosis of nervous system diseases. To evaluate the distribution of the protein NeuN in the motor cortex of normal and rabies-infected mice adult ICR mice were inoculated with rabies virus either intramuscularly or intracerebrally. Rabies-infected mice were sacrificed at the terminal stage of the disease. Control mice were also euthanized at the same age. The brains were removed and cut into coronal sections on a vibratome. Immunohistochemistry was used to study the expression of NeuN in the motor area of the cerebral cortex. Neuronal counts, cellular optical densitometry and neuronal diameter measurements were performed to analyze the immunoreactivity of the protein. All parameters revealed decreased immunoreactivity for NeuN in cortical neurons of mice intracerebrally infected with rabies. In contrast, the changes were not statistically significant in mice inoculated intramuscularly. Either the immunoreactivity of NeuN or its expression is affected by the presence of rabies virus in the cerebral cortex depending on the inoculation route. These results contribute to the knowledge of the dynamics of cellular infection on rabies pathogenesis.
author	Rengifo,Aura Caterine Umbarila,Vanessa Jazmin Garzón,Mary Janeth Torres-Fernández,Orlando
author_facet	Rengifo,Aura Caterine Umbarila,Vanessa Jazmin Garzón,Mary Janeth Torres-Fernández,Orlando
author_sort	Rengifo,Aura Caterine
title	Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
title_short	Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
title_full	Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
title_fullStr	Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
title_full_unstemmed	Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice
title_sort	differential effect of the route of inoculation of rabies virus on neun immunoreactivity in the cerebral cortex of mice
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400031
work_keys_str_mv	AT rengifoauracaterine differentialeffectoftherouteofinoculationofrabiesvirusonneunimmunoreactivityinthecerebralcortexofmice AT umbarilavanessajazmin differentialeffectoftherouteofinoculationofrabiesvirusonneunimmunoreactivityinthecerebralcortexofmice AT garzonmaryjaneth differentialeffectoftherouteofinoculationofrabiesvirusonneunimmunoreactivityinthecerebralcortexofmice AT torresfernandezorlando differentialeffectoftherouteofinoculationofrabiesvirusonneunimmunoreactivityinthecerebralcortexofmice
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Differential Effect of the Route of Inoculation of Rabies Virus on NeuN Immunoreactivity in the Cerebral Cortex of Mice

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