Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells

Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetu...

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Autores principales:	Chiapparrone,María L, Soto,Pedro, Catena,María
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Adhesion Campylobacter fetus subsp. venerealis Sperm cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040
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spelling	oai:scielo:S0717-950220160004000402017-02-01Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm CellsChiapparrone,María LSoto,PedroCatena,María Adhesion Campylobacter fetus subsp. venerealis Sperm cells Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetus subsp. venerealis when in interaction with bovine sperm cells. The bovine spermatozoa were obtained frozen bovine semen pooled from uninfected bulls, and were exposed to living campylobacter over different periods of time. Light microscopy and scanning electron microscopy first revealed a tropism, then a close proximity followed by tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed. Motile bacteria were observed during the next 3 h, this process began with a tight membranemembrane adhesion. The adhesion between Campylobacter fetus to the sperm cell occurred either by the flagella or by sperm head. Results from this study demonstrated with light microscopy scanning electron microscopy allowed us to characterize some aspects of the interaction of Campylobacter fetus subsp. venerealis and bovine sperm while preserving the cellular and bacterial structure. This ex vivo model might be useful for studies on adhesion and cytopathogenicity of different field strains of Campylobacter fetus.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040en10.4067/S0717-95022016000400040
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adhesion Campylobacter fetus subsp. venerealis Sperm cells
spellingShingle	Adhesion Campylobacter fetus subsp. venerealis Sperm cells Chiapparrone,María L Soto,Pedro Catena,María Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
description	Campylobacter fetus is extracellular bacteria of the genital tract of cattle. They cause infertility and abortion, but there is no documented information on the susceptibility of bovine sperm cells to this bacteria. The aim of this present work was to study the effects provoked by Campylobacter fetus subsp. venerealis when in interaction with bovine sperm cells. The bovine spermatozoa were obtained frozen bovine semen pooled from uninfected bulls, and were exposed to living campylobacter over different periods of time. Light microscopy and scanning electron microscopy first revealed a tropism, then a close proximity followed by tight adhesion between these two different cells. A decrease in the spermatozoa motility was observed. Motile bacteria were observed during the next 3 h, this process began with a tight membranemembrane adhesion. The adhesion between Campylobacter fetus to the sperm cell occurred either by the flagella or by sperm head. Results from this study demonstrated with light microscopy scanning electron microscopy allowed us to characterize some aspects of the interaction of Campylobacter fetus subsp. venerealis and bovine sperm while preserving the cellular and bacterial structure. This ex vivo model might be useful for studies on adhesion and cytopathogenicity of different field strains of Campylobacter fetus.
author	Chiapparrone,María L Soto,Pedro Catena,María
author_facet	Chiapparrone,María L Soto,Pedro Catena,María
author_sort	Chiapparrone,María L
title	Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
title_short	Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
title_full	Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
title_fullStr	Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
title_full_unstemmed	Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells
title_sort	characterization of the campylobacter fetus subsp. venerealis adhesion to bovine sperm cells
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400040
work_keys_str_mv	AT chiapparronemarial characterizationofthecampylobacterfetussubspvenerealisadhesiontobovinespermcells AT sotopedro characterizationofthecampylobacterfetussubspvenerealisadhesiontobovinespermcells AT catenamaria characterizationofthecampylobacterfetussubspvenerealisadhesiontobovinespermcells
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Characterization of the Campylobacter fetus subsp. venerealis Adhesion to Bovine Sperm Cells

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