Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin

Kinins are vasoactive peptides that promote pain and inflammation, yet centrally believed to participate to cardiovascular defensive reflexes produced by noxious stimuli. These peptides signal through the activation of two transmembrane G-protein-coupled receptors named B1 and B2 receptors (B1R and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	de França,Camilla Estevão, Guardabassi,Carla, Smith,Ricardo Luiz
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Medulla Spinal Cord Rats Kinin receptor B2 Capsaicin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400047
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022016000400047
record_format	dspace
spelling	oai:scielo:S0717-950220160004000472017-02-01Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicinde França,Camilla EstevãoGuardabassi,CarlaSmith,Ricardo Luiz Medulla Spinal Cord Rats Kinin receptor B2 Capsaicin Kinins are vasoactive peptides that promote pain and inflammation, yet centrally believed to participate to cardiovascular defensive reflexes produced by noxious stimuli. These peptides signal through the activation of two transmembrane G-protein-coupled receptors named B1 and B2 receptors (B1R and B2R). The B2R is constitutive in healthy tissues and animals. The aim of the study was to measure the gene and protein expression of B2R kinin receptors in central and peripheral tissues isolated from control rats and rats were pre-treated with capsaicin on the second day of life (50 mg/kg, s.c.) or two weeks prior to sacrifice (125 mg/kg over three days, s.c.). The same treatment with saline was made in control animals. Levels of mRNA for B2R were measured by quantitative RT-PCR and Qualitative while receptor binding sites were measured on tissue sections with the radioligands 125I-HPP-Hoe 140 (B2R). B2R was expressed in all studied tissues (hypothalamus, paratrigeminal nucleus, nucleus of solitary tract, spinal cord, aorta and liver) and treatment capsaicin neonates when compared to controls, did not affect its level of expression. Capsaicin had no significant effect on the expression of B2R in some tissues on binding sites. The synthesis of B2R kinin receptor is not associated with sensory C-fibre and tissues showed no significant difference indicating that B2R was regulated by distinct mechanisms.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400047en10.4067/S0717-95022016000400047
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Medulla Spinal Cord Rats Kinin receptor B2 Capsaicin
spellingShingle	Medulla Spinal Cord Rats Kinin receptor B2 Capsaicin de França,Camilla Estevão Guardabassi,Carla Smith,Ricardo Luiz Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
description	Kinins are vasoactive peptides that promote pain and inflammation, yet centrally believed to participate to cardiovascular defensive reflexes produced by noxious stimuli. These peptides signal through the activation of two transmembrane G-protein-coupled receptors named B1 and B2 receptors (B1R and B2R). The B2R is constitutive in healthy tissues and animals. The aim of the study was to measure the gene and protein expression of B2R kinin receptors in central and peripheral tissues isolated from control rats and rats were pre-treated with capsaicin on the second day of life (50 mg/kg, s.c.) or two weeks prior to sacrifice (125 mg/kg over three days, s.c.). The same treatment with saline was made in control animals. Levels of mRNA for B2R were measured by quantitative RT-PCR and Qualitative while receptor binding sites were measured on tissue sections with the radioligands 125I-HPP-Hoe 140 (B2R). B2R was expressed in all studied tissues (hypothalamus, paratrigeminal nucleus, nucleus of solitary tract, spinal cord, aorta and liver) and treatment capsaicin neonates when compared to controls, did not affect its level of expression. Capsaicin had no significant effect on the expression of B2R in some tissues on binding sites. The synthesis of B2R kinin receptor is not associated with sensory C-fibre and tissues showed no significant difference indicating that B2R was regulated by distinct mechanisms.
author	de França,Camilla Estevão Guardabassi,Carla Smith,Ricardo Luiz
author_facet	de França,Camilla Estevão Guardabassi,Carla Smith,Ricardo Luiz
author_sort	de França,Camilla Estevão
title	Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
title_short	Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
title_full	Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
title_fullStr	Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
title_full_unstemmed	Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin
title_sort	expression and distribution of kinin b2 receptor in medulla and spinal cord tissues of rats treated with capsaicin
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400047
work_keys_str_mv	AT defrancacamillaestevao expressionanddistributionofkininb2receptorinmedullaandspinalcordtissuesofratstreatedwithcapsaicin AT guardabassicarla expressionanddistributionofkininb2receptorinmedullaandspinalcordtissuesofratstreatedwithcapsaicin AT smithricardoluiz expressionanddistributionofkininb2receptorinmedullaandspinalcordtissuesofratstreatedwithcapsaicin
_version_	1718444985590743040

Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin

Ejemplares similares