Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quan...

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Autores principales:	Góis,Marcelo Biondaro, Hermes-Uliana,Catchia, Paltanin,Alessandra, Pontes,Willian, Araújo,Eduardo José De Almeida, Miranda Neto,Marcilio Hubner, Sant'Ana,Débora Mello Gonçales
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2016
Materias:	Enteric nervous system Meissner's plexus Giemsa NADH-diaphorase NADPH-diaphorase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050
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spelling	oai:scielo:S0717-950220160004000502017-02-01Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical TechniquesGóis,Marcelo BiondaroHermes-Uliana,CatchiaPaltanin,AlessandraPontes,WillianAraújo,Eduardo José De AlmeidaMiranda Neto,Marcilio HubnerSant'Ana,Débora Mello Gonçales Enteric nervous system Meissner's plexus Giemsa NADH-diaphorase NADPH-diaphorase Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the submucosal plexus of adult rats are mainly organized in ganglia with varied sized and shapes. Giemsa technique stained 243.93 ± 7.68 neurons per mm2. Regarding the total population stained by Giemsa, NADH- diaphorase positive (139.09 ± 11.14/mm2) neurons represented 57 % and NADPH-diaphorase positive (18.17 ± 0.28/mm2) represented 7.5 %. The area of the cell body was bigger in nitrergic neurons (412.29 ± 150.22) than in the ones stained by Giemsa (254.71 ± 63.32) and NADH-diaphorase positive (243.98 ± 123.82).info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.34 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050en10.4067/S0717-95022016000400050
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enteric nervous system Meissner's plexus Giemsa NADH-diaphorase NADPH-diaphorase
spellingShingle	Enteric nervous system Meissner's plexus Giemsa NADH-diaphorase NADPH-diaphorase Góis,Marcelo Biondaro Hermes-Uliana,Catchia Paltanin,Alessandra Pontes,Willian Araújo,Eduardo José De Almeida Miranda Neto,Marcilio Hubner Sant'Ana,Débora Mello Gonçales Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
description	Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the submucosal plexus of adult rats are mainly organized in ganglia with varied sized and shapes. Giemsa technique stained 243.93 ± 7.68 neurons per mm2. Regarding the total population stained by Giemsa, NADH- diaphorase positive (139.09 ± 11.14/mm2) neurons represented 57 % and NADPH-diaphorase positive (18.17 ± 0.28/mm2) represented 7.5 %. The area of the cell body was bigger in nitrergic neurons (412.29 ± 150.22) than in the ones stained by Giemsa (254.71 ± 63.32) and NADH-diaphorase positive (243.98 ± 123.82).
author	Góis,Marcelo Biondaro Hermes-Uliana,Catchia Paltanin,Alessandra Pontes,Willian Araújo,Eduardo José De Almeida Miranda Neto,Marcilio Hubner Sant'Ana,Débora Mello Gonçales
author_facet	Góis,Marcelo Biondaro Hermes-Uliana,Catchia Paltanin,Alessandra Pontes,Willian Araújo,Eduardo José De Almeida Miranda Neto,Marcilio Hubner Sant'Ana,Débora Mello Gonçales
author_sort	Góis,Marcelo Biondaro
title	Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
title_short	Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
title_full	Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
title_fullStr	Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
title_full_unstemmed	Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques
title_sort	morphoquantitative study of rattus norvegicus submucosal plexus by different neuronal evidentiation histochemical techniques
publisher	Sociedad Chilena de Anatomía
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050
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Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques

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