Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring

Several studies indicated that pancreatic ß-cell death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the ß-cells in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated...

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Autores principales:	Nazari,Zahra, Nabiuni,Mohammad, Ghaffari,Soraya, Saeidi,Mohsen, Shahriyari,Alireza, Golalipour,Mohammad Jafar
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Gestational diabetes mellitus Pancreatic islets (Langerhans) ß-cell Apoptosis Rat offspring
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100003
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spelling	oai:scielo:S0717-950220170001000032017-04-18Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat OffspringNazari,ZahraNabiuni,MohammadGhaffari,SorayaSaeidi,MohsenShahriyari,AlirezaGolalipour,Mohammad Jafar Gestational diabetes mellitus Pancreatic islets (Langerhans) ß-cell Apoptosis Rat offspring Several studies indicated that pancreatic ß-cell death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the ß-cells in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of ß-cells and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in ß-cell number in offspring of GDM (p<0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P<0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100003en10.4067/S0717-95022017000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gestational diabetes mellitus Pancreatic islets (Langerhans) ß-cell Apoptosis Rat offspring
spellingShingle	Gestational diabetes mellitus Pancreatic islets (Langerhans) ß-cell Apoptosis Rat offspring Nazari,Zahra Nabiuni,Mohammad Ghaffari,Soraya Saeidi,Mohsen Shahriyari,Alireza Golalipour,Mohammad Jafar Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
description	Several studies indicated that pancreatic ß-cell death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the ß-cells in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of ß-cells and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in ß-cell number in offspring of GDM (p<0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P<0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring.
author	Nazari,Zahra Nabiuni,Mohammad Ghaffari,Soraya Saeidi,Mohsen Shahriyari,Alireza Golalipour,Mohammad Jafar
author_facet	Nazari,Zahra Nabiuni,Mohammad Ghaffari,Soraya Saeidi,Mohsen Shahriyari,Alireza Golalipour,Mohammad Jafar
author_sort	Nazari,Zahra
title	Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
title_short	Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
title_full	Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
title_fullStr	Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
title_full_unstemmed	Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring
title_sort	gestational diabetes induces pancreatic beta-cells apoptosis in adult rat offspring
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100003
work_keys_str_mv	AT nazarizahra gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring AT nabiunimohammad gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring AT ghaffarisoraya gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring AT saeidimohsen gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring AT shahriyarialireza gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring AT golalipourmohammadjafar gestationaldiabetesinducespancreaticbetacellsapoptosisinadultratoffspring
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Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring

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