Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors

The combined antiretroviral therapy (cART), a multidrug combination regimen, usually consisting Nucleoside Reverse Transcriptase Inhibitors, non- Nucleoside Reverse Transcriptase Inhibitors and Protease Inhibitors has altered the morbidity pattern affecting HIV-infected individuals to include non-AI...

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Autores principales:	Adefolaju,Gbenga Anthony, Scholtz,Kathrine Elizabeth, Hosie,Margot Jill
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Reverse Transcriptase Inhibitors Protease Inhibitors VEGF165b Breast cancer MCF-7 MCF-10A
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100024
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spelling	oai:scielo:S0717-950220170001000242017-04-18Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease InhibitorsAdefolaju,Gbenga AnthonyScholtz,Kathrine ElizabethHosie,Margot Jill Reverse Transcriptase Inhibitors Protease Inhibitors VEGF165b Breast cancer MCF-7 MCF-10A The combined antiretroviral therapy (cART), a multidrug combination regimen, usually consisting Nucleoside Reverse Transcriptase Inhibitors, non- Nucleoside Reverse Transcriptase Inhibitors and Protease Inhibitors has altered the morbidity pattern affecting HIV-infected individuals to include non-AIDS-defining malignancies (nADMs). The speculation is rife; does cART induce or promote the progression of nADMs such as breast cancer? This study was therefore designed to investigate of the effects of some antiretroviral drugs (at clinically relevant concentrations) on the expression of anti-angiogenic gene; VEGF165b in two human breast cell lines; MCF-7 and MCF-10A by Real Time qPCR and immuno-fluorescence. All of the antiretroviral drugs and combinations tested produced patterns of slight up or downregulation of VEGF165b mRNA expression but the alterations did not attain statistical significance. They also did not alter VEGF165bprotein localisation in both cell lines. The findings reported here suggest that antiretroviral drugs probably do not influence the angiogenic pathway in the development of breast cancer in patients under the combined antiretroviral regimen.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100024en10.4067/S0717-95022017000100024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Reverse Transcriptase Inhibitors Protease Inhibitors VEGF165b Breast cancer MCF-7 MCF-10A
spellingShingle	Reverse Transcriptase Inhibitors Protease Inhibitors VEGF165b Breast cancer MCF-7 MCF-10A Adefolaju,Gbenga Anthony Scholtz,Kathrine Elizabeth Hosie,Margot Jill Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
description	The combined antiretroviral therapy (cART), a multidrug combination regimen, usually consisting Nucleoside Reverse Transcriptase Inhibitors, non- Nucleoside Reverse Transcriptase Inhibitors and Protease Inhibitors has altered the morbidity pattern affecting HIV-infected individuals to include non-AIDS-defining malignancies (nADMs). The speculation is rife; does cART induce or promote the progression of nADMs such as breast cancer? This study was therefore designed to investigate of the effects of some antiretroviral drugs (at clinically relevant concentrations) on the expression of anti-angiogenic gene; VEGF165b in two human breast cell lines; MCF-7 and MCF-10A by Real Time qPCR and immuno-fluorescence. All of the antiretroviral drugs and combinations tested produced patterns of slight up or downregulation of VEGF165b mRNA expression but the alterations did not attain statistical significance. They also did not alter VEGF165bprotein localisation in both cell lines. The findings reported here suggest that antiretroviral drugs probably do not influence the angiogenic pathway in the development of breast cancer in patients under the combined antiretroviral regimen.
author	Adefolaju,Gbenga Anthony Scholtz,Kathrine Elizabeth Hosie,Margot Jill
author_facet	Adefolaju,Gbenga Anthony Scholtz,Kathrine Elizabeth Hosie,Margot Jill
author_sort	Adefolaju,Gbenga Anthony
title	Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
title_short	Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
title_full	Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
title_fullStr	Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
title_full_unstemmed	Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors
title_sort	antiangiogenic vegf165b expression in human breast mcf-7 and mcf-10a cells exposed to reverse transcriptase and protease inhibitors
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100024
work_keys_str_mv	AT adefolajugbengaanthony antiangiogenicvegf165bexpressioninhumanbreastmcf7andmcf10acellsexposedtoreversetranscriptaseandproteaseinhibitors AT scholtzkathrineelizabeth antiangiogenicvegf165bexpressioninhumanbreastmcf7andmcf10acellsexposedtoreversetranscriptaseandproteaseinhibitors AT hosiemargotjill antiangiogenicvegf165bexpressioninhumanbreastmcf7andmcf10acellsexposedtoreversetranscriptaseandproteaseinhibitors
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Antiangiogenic VEGF165b Expression in Human Breast MCF-7 and MCF-10A Cells Exposed to Reverse Transcriptase and Protease Inhibitors

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