Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations

Human cerebellum is important for motor coordination; muscle tones and maintaing the equilibrium of the body. In our region, limited data is available on the normal morphology of human cerebellum, whilst fresh biopsy is quite difficult to obtain. Here adult male cerebellum from cadaver anatomy prepa...

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Autores principales:	Kalanjati,V. P, Dewi,A. K, Santoso,M. W. A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Metencephalon Cerebelar cortex Measurement Structure
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100027
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spelling	oai:scielo:S0717-950220170001000272017-04-18Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver PreparationsKalanjati,V. PDewi,A. KSantoso,M. W. A Metencephalon Cerebelar cortex Measurement Structure Human cerebellum is important for motor coordination; muscle tones and maintaing the equilibrium of the body. In our region, limited data is available on the normal morphology of human cerebellum, whilst fresh biopsy is quite difficult to obtain. Here adult male cerebellum from cadaver anatomy preparations embalmed with 37 % formalin fixative solution for over a year are studied (n=3). After removal, right cerebellum hemisphere was sliced into cubicle then temporary soaked into 50-60 % of alcohol before being paraffinated. Two parasagittal adjacent slices from each sample were deparaffinated (5 µm) and then stained with hematoxyllin-eosin (HE). Slides were observed under light microscope (Olympus, Japan). Pictures were analysed from 6 field numbers of each, with Optilab and Image Raster 3 software (Indonesia). The density of the Purkinje cells (Neuron purkinjense), the number and density of the Purkinje cells and the thickness of the molecular layer are measured. Data were analysed with the level of significance of p<0.05 (ANOVA, Microsoft Excel 2007). The distance between 2 Purkinje cells is ranged between 82.6-346.6 µm, although no significant differences found (p=0.1). There are no significant differences in the number and in the density of the Purkinje cells amongst samples (p=0.72 and 0.34, respectively); might be due to the similar age, sex and race of these cadavers. However, there is a significant difference in the thickness of the molecular layer (p=0.015). Variations amongst individual cerebelli are observed, with a significant different thickness in the molecular layer. The cellular composition of each cerebellum is unique, arguably correlated to the individual cerebellum activity when alive.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100027en10.4067/S0717-95022017000100027
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Metencephalon Cerebelar cortex Measurement Structure
spellingShingle	Metencephalon Cerebelar cortex Measurement Structure Kalanjati,V. P Dewi,A. K Santoso,M. W. A Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
description	Human cerebellum is important for motor coordination; muscle tones and maintaing the equilibrium of the body. In our region, limited data is available on the normal morphology of human cerebellum, whilst fresh biopsy is quite difficult to obtain. Here adult male cerebellum from cadaver anatomy preparations embalmed with 37 % formalin fixative solution for over a year are studied (n=3). After removal, right cerebellum hemisphere was sliced into cubicle then temporary soaked into 50-60 % of alcohol before being paraffinated. Two parasagittal adjacent slices from each sample were deparaffinated (5 µm) and then stained with hematoxyllin-eosin (HE). Slides were observed under light microscope (Olympus, Japan). Pictures were analysed from 6 field numbers of each, with Optilab and Image Raster 3 software (Indonesia). The density of the Purkinje cells (Neuron purkinjense), the number and density of the Purkinje cells and the thickness of the molecular layer are measured. Data were analysed with the level of significance of p<0.05 (ANOVA, Microsoft Excel 2007). The distance between 2 Purkinje cells is ranged between 82.6-346.6 µm, although no significant differences found (p=0.1). There are no significant differences in the number and in the density of the Purkinje cells amongst samples (p=0.72 and 0.34, respectively); might be due to the similar age, sex and race of these cadavers. However, there is a significant difference in the thickness of the molecular layer (p=0.015). Variations amongst individual cerebelli are observed, with a significant different thickness in the molecular layer. The cellular composition of each cerebellum is unique, arguably correlated to the individual cerebellum activity when alive.
author	Kalanjati,V. P Dewi,A. K Santoso,M. W. A
author_facet	Kalanjati,V. P Dewi,A. K Santoso,M. W. A
author_sort	Kalanjati,V. P
title	Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
title_short	Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
title_full	Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
title_fullStr	Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
title_full_unstemmed	Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations
title_sort	quantitative study on human cerebellar cortex from anatomy cadaver preparations
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100027
work_keys_str_mv	AT kalanjativp quantitativestudyonhumancerebellarcortexfromanatomycadaverpreparations AT dewiak quantitativestudyonhumancerebellarcortexfromanatomycadaverpreparations AT santosomwa quantitativestudyonhumancerebellarcortexfromanatomycadaverpreparations
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Quantitative Study on Human Cerebellar Cortex from Anatomy Cadaver Preparations

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