Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach
The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.)...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2017
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oai:scielo:S0717-950220170001000292017-04-18Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approachda Silva,Jairo Pinheirode Carvalho,Camila Albuquerque MelloRomualdo,Andressa RodriguesSchiavoni,Vagner SarraipoBarbosa,Marcello Henrique Nogueirade Moraes,Marcella Suélen TorrecillaTirapelli,Daniela Petri CunhaFazan,Valéria de Paula SassoliThomazini,José AntônioTirapelli,Luis Fernando Bone defect Diabetes mellitus Laser therapy Regeneration Apoptosis The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w 4 J/cm and 100 w 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone defect area and volume in both laser treated groups, control and diabetic. Therefore the low intensity laser therapy was effective in accelerating bone repair in both, control and diabetic groups. It was evidenced in our study that diabetes influences bone repair negatively.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100029en10.4067/S0717-95022017000100029 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Bone defect Diabetes mellitus Laser therapy Regeneration Apoptosis |
| spellingShingle |
Bone defect Diabetes mellitus Laser therapy Regeneration Apoptosis da Silva,Jairo Pinheiro de Carvalho,Camila Albuquerque Mello Romualdo,Andressa Rodrigues Schiavoni,Vagner Sarraipo Barbosa,Marcello Henrique Nogueira de Moraes,Marcella Suélen Torrecilla Tirapelli,Daniela Petri Cunha Fazan,Valéria de Paula Sassoli Thomazini,José Antônio Tirapelli,Luis Fernando Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| description |
The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w 4 J/cm and 100 w 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone defect area and volume in both laser treated groups, control and diabetic. Therefore the low intensity laser therapy was effective in accelerating bone repair in both, control and diabetic groups. It was evidenced in our study that diabetes influences bone repair negatively. |
| author |
da Silva,Jairo Pinheiro de Carvalho,Camila Albuquerque Mello Romualdo,Andressa Rodrigues Schiavoni,Vagner Sarraipo Barbosa,Marcello Henrique Nogueira de Moraes,Marcella Suélen Torrecilla Tirapelli,Daniela Petri Cunha Fazan,Valéria de Paula Sassoli Thomazini,José Antônio Tirapelli,Luis Fernando |
| author_facet |
da Silva,Jairo Pinheiro de Carvalho,Camila Albuquerque Mello Romualdo,Andressa Rodrigues Schiavoni,Vagner Sarraipo Barbosa,Marcello Henrique Nogueira de Moraes,Marcella Suélen Torrecilla Tirapelli,Daniela Petri Cunha Fazan,Valéria de Paula Sassoli Thomazini,José Antônio Tirapelli,Luis Fernando |
| author_sort |
da Silva,Jairo Pinheiro |
| title |
Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| title_short |
Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| title_full |
Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| title_fullStr |
Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| title_full_unstemmed |
Apoptosis in Bone Defect of Diabetic Rats Treated with Low Intensity Laser: Radiological and Immunohistochemical Approach |
| title_sort |
apoptosis in bone defect of diabetic rats treated with low intensity laser: radiological and immunohistochemical approach |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2017 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100029 |
| work_keys_str_mv |
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| _version_ |
1718444992748322816 |