In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo

The aim of this study was to observe morphological changes of the cultured otocysts isolated from various stages of the chick embryo. Isolated otocysts were dissected from embryonic day, E2.5-4.5 of incubation (HH stage 16-26) according to stages of developing inner ear. Morphology of the chick otoc...

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Autores principales:	Intarapat,Sittipon, Gonmanee,Thanasup, Thonabulsombat,Charoensri
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Chick embryo Inner ear Otocyst Otic development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100034
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spelling	oai:scielo:S0717-950220170001000342017-04-18In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick EmbryoIntarapat,SittiponGonmanee,ThanasupThonabulsombat,Charoensri Chick embryo Inner ear Otocyst Otic development The aim of this study was to observe morphological changes of the cultured otocysts isolated from various stages of the chick embryo. Isolated otocysts were dissected from embryonic day, E2.5-4.5 of incubation (HH stage 16-26) according to stages of developing inner ear. Morphology of the chick otocyst exhibited an ovoid shape. The width and height of the otocyst were 0.2 mm and 0.3 mm, respectively. Elongation of a tube-like structure, the endolymphatic duct, was found at the dorsal aspect of the otocyst. The cultured otocyst is lined by the otic epithelium and surrounding periotic mesenchymal cells started to migrate outwards the lateral aspect of such epithelium. Notably, the acoustic-vestibular ganglion (AVG) was observed at the ventrolateral aspect of the otocyst. Appearance of AVG in vitro can be applied for studying chemical-induced ototoxicity and sensorineural hearing loss. It was concluded that the organ-cultured otocyst of the chick embryo could be used as a model to study sensory organ development of avian inner ear.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100034en10.4067/S0717-95022017000100034
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chick embryo Inner ear Otocyst Otic development
spellingShingle	Chick embryo Inner ear Otocyst Otic development Intarapat,Sittipon Gonmanee,Thanasup Thonabulsombat,Charoensri In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
description	The aim of this study was to observe morphological changes of the cultured otocysts isolated from various stages of the chick embryo. Isolated otocysts were dissected from embryonic day, E2.5-4.5 of incubation (HH stage 16-26) according to stages of developing inner ear. Morphology of the chick otocyst exhibited an ovoid shape. The width and height of the otocyst were 0.2 mm and 0.3 mm, respectively. Elongation of a tube-like structure, the endolymphatic duct, was found at the dorsal aspect of the otocyst. The cultured otocyst is lined by the otic epithelium and surrounding periotic mesenchymal cells started to migrate outwards the lateral aspect of such epithelium. Notably, the acoustic-vestibular ganglion (AVG) was observed at the ventrolateral aspect of the otocyst. Appearance of AVG in vitro can be applied for studying chemical-induced ototoxicity and sensorineural hearing loss. It was concluded that the organ-cultured otocyst of the chick embryo could be used as a model to study sensory organ development of avian inner ear.
author	Intarapat,Sittipon Gonmanee,Thanasup Thonabulsombat,Charoensri
author_facet	Intarapat,Sittipon Gonmanee,Thanasup Thonabulsombat,Charoensri
author_sort	Intarapat,Sittipon
title	In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
title_short	In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
title_full	In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
title_fullStr	In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
title_full_unstemmed	In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo
title_sort	in vitro study of morphological changes of the cultured otocyst isolated from the chick embryo
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100034
work_keys_str_mv	AT intarapatsittipon invitrostudyofmorphologicalchangesoftheculturedotocystisolatedfromthechickembryo AT gonmaneethanasup invitrostudyofmorphologicalchangesoftheculturedotocystisolatedfromthechickembryo AT thonabulsombatcharoensri invitrostudyofmorphologicalchangesoftheculturedotocystisolatedfromthechickembryo
_version_	1718444993658486784

In vitro Study of Morphological Changes of the Cultured Otocyst Isolated from the Chick Embryo

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