Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim...

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Autores principales:	Bahmani,Tahere, Gholami,Samira, Mostafaie,Ali, Karimi,Ebrahim, Hoseinkhani,Zohreh, Azad,Taha, Mahdizadeh,Hamid, Mansouri,Kamran
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Olfactory mucosa Neural stem cells Hair cells Differentiation Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200004
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spelling	oai:scielo:S0717-950220170002000042017-08-07Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair CellsBahmani,TahereGholami,SamiraMostafaie,AliKarimi,EbrahimHoseinkhani,ZohrehAzad,TahaMahdizadeh,HamidMansouri,Kamran Olfactory mucosa Neural stem cells Hair cells Differentiation Rat Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other and placed in flask containing defined differentiation medium. Cells at this stage cultured in adhesive form. Immunohistochemistry and RT-PCR were used for neural stem cells and hair cells identification. Spheres formed from olfactory epithelium culture and immunohistochemistry revealed that cells of spheres from passage one and two expressed the neural stem cells markers. After culture of isolated cells in differentiation medium, the morphology of cells begun to change. The cells presented neural cells projections and after 10 days the projections elongated more and interact to each other in multi layers. RT-PCR and immunohistochemistry revealed that differentiated cells expressed hair cells specific genes. In this study we showed that neural stem cells of olfactory epithelium can differentiate into hair cells of inner ear and therefore can be used for treatment of SNHL.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200004en10.4067/S0717-95022017000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Olfactory mucosa Neural stem cells Hair cells Differentiation Rat
spellingShingle	Olfactory mucosa Neural stem cells Hair cells Differentiation Rat Bahmani,Tahere Gholami,Samira Mostafaie,Ali Karimi,Ebrahim Hoseinkhani,Zohreh Azad,Taha Mahdizadeh,Hamid Mansouri,Kamran Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
description	Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other and placed in flask containing defined differentiation medium. Cells at this stage cultured in adhesive form. Immunohistochemistry and RT-PCR were used for neural stem cells and hair cells identification. Spheres formed from olfactory epithelium culture and immunohistochemistry revealed that cells of spheres from passage one and two expressed the neural stem cells markers. After culture of isolated cells in differentiation medium, the morphology of cells begun to change. The cells presented neural cells projections and after 10 days the projections elongated more and interact to each other in multi layers. RT-PCR and immunohistochemistry revealed that differentiated cells expressed hair cells specific genes. In this study we showed that neural stem cells of olfactory epithelium can differentiate into hair cells of inner ear and therefore can be used for treatment of SNHL.
author	Bahmani,Tahere Gholami,Samira Mostafaie,Ali Karimi,Ebrahim Hoseinkhani,Zohreh Azad,Taha Mahdizadeh,Hamid Mansouri,Kamran
author_facet	Bahmani,Tahere Gholami,Samira Mostafaie,Ali Karimi,Ebrahim Hoseinkhani,Zohreh Azad,Taha Mahdizadeh,Hamid Mansouri,Kamran
author_sort	Bahmani,Tahere
title	Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
title_short	Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
title_full	Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
title_fullStr	Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
title_full_unstemmed	Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
title_sort	olfactory epithelium as an infinitive source of neural stem cells for derivation of inner ear hair cells
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200004
work_keys_str_mv	AT bahmanitahere olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT gholamisamira olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT mostafaieali olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT karimiebrahim olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT hoseinkhanizohreh olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT azadtaha olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT mahdizadehhamid olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells AT mansourikamran olfactoryepitheliumasaninfinitivesourceofneuralstemcellsforderivationofinnerearhaircells
_version_	1718444998970572800

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells

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