Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone
The aim of the present study was to observe the changes in the muscle tissue of rats after application of growth hormone (GH) and performing a strength training protocol (ST). In total, 40 male Wistar rats, 60 days old, were used, divided into four groups: control (C), control and application of GH...
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Sociedad Chilena de Anatomía
2017
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oai:scielo:S0717-950220170002000152017-08-07Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth HormoneCastoldi,Robson ChaconOzaki,Guilherme Akio TamuraGiometti,Ines CristinaCamargo,Regina Celi TrindadeEmyKoike,TatianaGarcia,Thiago AlvesFilho,José Carlos Silva CamargoBelangero,William Dias Growth hormone Striated Muscle Orthopedics Physical Education and Training Endocrinology The aim of the present study was to observe the changes in the muscle tissue of rats after application of growth hormone (GH) and performing a strength training protocol (ST). In total, 40 male Wistar rats, 60 days old, were used, divided into four groups: control (C), control and application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The physical training protocol (PT) was composed of four series of 10 jumps in water, 3x/week, with an overload of 50 % of body weight for four weeks. GH was administered intraperitoneally at a dose of 0.2 IU/Kg to the GHC and GHT groups and saline (0.9 % sodium chloride) to the C and T groups. After four weeks of PT, the animals were euthanized and samples taken from the Soleus muscle. Histological sections were produced with a thickness of 5 mm and stained with hematoxylin-eosin (HE) and nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR). The markings for determining the measurement of the smallest diameter of muscle fibers (MF) were carried out using the software (AuxioVisionRel 4.8-Carl Zeiss® and NIS-Elements D3.0-SP7-Nikon®). After obtaining the data, the Shapiro-Wilk test for normality was performed and then the nonparametric Kruskal-Wallis with Dunn post-test were used for analysis of MF and the Student t test for the analysis of intragroup body weights. All procedures adopted a 5 % significance value (p <0.05) and were performed using the software SPSS 22.0 for Windows®. It was observed that both the GH and PT were able to generate increased diameter of MF (C:31.81±6.35; GHC:36.88±6.38; T:38.38± 6.94; GHT:36.89±7.16). Moreover, when analyzing the type, a significant increase was found only in the fast twitch MF (C:33.78±7.78; GHC:37.80±6.03; T:38.53±6.94; GHT:37.98±7.65) when compared to the slow twitch (C:25.93±6.66; GHC:26.95±8.03; T:26.24±6.90; GHT:27.20±5.77).info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200015en10.4067/S0717-95022017000200015 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Growth hormone Striated Muscle Orthopedics Physical Education and Training Endocrinology |
| spellingShingle |
Growth hormone Striated Muscle Orthopedics Physical Education and Training Endocrinology Castoldi,Robson Chacon Ozaki,Guilherme Akio Tamura Giometti,Ines Cristina Camargo,Regina Celi Trindade EmyKoike,Tatiana Garcia,Thiago Alves Filho,José Carlos Silva Camargo Belangero,William Dias Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| description |
The aim of the present study was to observe the changes in the muscle tissue of rats after application of growth hormone (GH) and performing a strength training protocol (ST). In total, 40 male Wistar rats, 60 days old, were used, divided into four groups: control (C), control and application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The physical training protocol (PT) was composed of four series of 10 jumps in water, 3x/week, with an overload of 50 % of body weight for four weeks. GH was administered intraperitoneally at a dose of 0.2 IU/Kg to the GHC and GHT groups and saline (0.9 % sodium chloride) to the C and T groups. After four weeks of PT, the animals were euthanized and samples taken from the Soleus muscle. Histological sections were produced with a thickness of 5 mm and stained with hematoxylin-eosin (HE) and nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR). The markings for determining the measurement of the smallest diameter of muscle fibers (MF) were carried out using the software (AuxioVisionRel 4.8-Carl Zeiss® and NIS-Elements D3.0-SP7-Nikon®). After obtaining the data, the Shapiro-Wilk test for normality was performed and then the nonparametric Kruskal-Wallis with Dunn post-test were used for analysis of MF and the Student t test for the analysis of intragroup body weights. All procedures adopted a 5 % significance value (p <0.05) and were performed using the software SPSS 22.0 for Windows®. It was observed that both the GH and PT were able to generate increased diameter of MF (C:31.81±6.35; GHC:36.88±6.38; T:38.38± 6.94; GHT:36.89±7.16). Moreover, when analyzing the type, a significant increase was found only in the fast twitch MF (C:33.78±7.78; GHC:37.80±6.03; T:38.53±6.94; GHT:37.98±7.65) when compared to the slow twitch (C:25.93±6.66; GHC:26.95±8.03; T:26.24±6.90; GHT:27.20±5.77). |
| author |
Castoldi,Robson Chacon Ozaki,Guilherme Akio Tamura Giometti,Ines Cristina Camargo,Regina Celi Trindade EmyKoike,Tatiana Garcia,Thiago Alves Filho,José Carlos Silva Camargo Belangero,William Dias |
| author_facet |
Castoldi,Robson Chacon Ozaki,Guilherme Akio Tamura Giometti,Ines Cristina Camargo,Regina Celi Trindade EmyKoike,Tatiana Garcia,Thiago Alves Filho,José Carlos Silva Camargo Belangero,William Dias |
| author_sort |
Castoldi,Robson Chacon |
| title |
Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| title_short |
Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| title_full |
Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| title_fullStr |
Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| title_full_unstemmed |
Morphometric Study of Muscle Fibers in Rats Submitted to Strength Training and Growth Hormone |
| title_sort |
morphometric study of muscle fibers in rats submitted to strength training and growth hormone |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2017 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200015 |
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