Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat

When a vein segment is grafted into arterial circulation, biomechanical forces stimulate modification of its structure. This morphological adaptive response is progressive during a medium or long term and occludes the vessel lumen, leading to a graft failure. The objective of this study was to chara...

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Autores principales:	Velazquez-Gauna,Sergio E, Soto-Domínguez,Adolfo, Quiroga-Garza,Alejandro, Reyes-Hernández,Cynthia G, Chavez-Reyes,Arturo, Morales-Avalos,Rodolfo, Montes-de-Oca-Luna,Roberto, Guzmán-López,Santos, Elizondo-Omaña,Rodrigo E
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Hyperplasia Morphology Microsurgical model Vascular adaptive changes Venous graft
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200016
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spelling	oai:scielo:S0717-950220170002000162017-08-07Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar RatVelazquez-Gauna,Sergio ESoto-Domínguez,AdolfoQuiroga-Garza,AlejandroReyes-Hernández,Cynthia GChavez-Reyes,ArturoMorales-Avalos,RodolfoMontes-de-Oca-Luna,RobertoGuzmán-López,SantosElizondo-Omaña,Rodrigo E Hyperplasia Morphology Microsurgical model Vascular adaptive changes Venous graft When a vein segment is grafted into arterial circulation, biomechanical forces stimulate modification of its structure. This morphological adaptive response is progressive during a medium or long term and occludes the vessel lumen, leading to a graft failure. The objective of this study was to characterize the early morphological response of the vascular wall in a terminal-terminal vascular vein graft model in Wistar rats. A segment of the femoral vein was placed in the femoral circulation. An end to end microsurgical graft anastomosis technique was implemented and standardized in twenty rats. The samples were processed with histological technique to analyze the overall structure with hematoxylin and eosin, the composition of the vessel wall with Masson trichrome technique, the proliferating and smooth muscle cells were detected with immunohistochemistry (anti-PCNA, anti-actin and anti CD68) and the induction of apoptosis with TUNEL technique. The times periods studied were 1, 3 and 5 days postoperative. There is progressive increase of cell proliferation and intensity of the density detected by PCNA with its peak at postoperative day 3. Apoptosis was not evident in any of the postoperative days. Smooth muscle had no significant change in any of the time periods studied. Macrophage and leukocyte migration was evident since the first postoperative day with infiltration into the media by the 5th day. This study characterizes the morphological aspects in the early arterialization of the vascular wall in a vein graft process. These results contribute to a better understanding of the morphopathological mechanism involved in vein graft failure.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200016en10.4067/S0717-95022017000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hyperplasia Morphology Microsurgical model Vascular adaptive changes Venous graft
spellingShingle	Hyperplasia Morphology Microsurgical model Vascular adaptive changes Venous graft Velazquez-Gauna,Sergio E Soto-Domínguez,Adolfo Quiroga-Garza,Alejandro Reyes-Hernández,Cynthia G Chavez-Reyes,Arturo Morales-Avalos,Rodolfo Montes-de-Oca-Luna,Roberto Guzmán-López,Santos Elizondo-Omaña,Rodrigo E Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
description	When a vein segment is grafted into arterial circulation, biomechanical forces stimulate modification of its structure. This morphological adaptive response is progressive during a medium or long term and occludes the vessel lumen, leading to a graft failure. The objective of this study was to characterize the early morphological response of the vascular wall in a terminal-terminal vascular vein graft model in Wistar rats. A segment of the femoral vein was placed in the femoral circulation. An end to end microsurgical graft anastomosis technique was implemented and standardized in twenty rats. The samples were processed with histological technique to analyze the overall structure with hematoxylin and eosin, the composition of the vessel wall with Masson trichrome technique, the proliferating and smooth muscle cells were detected with immunohistochemistry (anti-PCNA, anti-actin and anti CD68) and the induction of apoptosis with TUNEL technique. The times periods studied were 1, 3 and 5 days postoperative. There is progressive increase of cell proliferation and intensity of the density detected by PCNA with its peak at postoperative day 3. Apoptosis was not evident in any of the postoperative days. Smooth muscle had no significant change in any of the time periods studied. Macrophage and leukocyte migration was evident since the first postoperative day with infiltration into the media by the 5th day. This study characterizes the morphological aspects in the early arterialization of the vascular wall in a vein graft process. These results contribute to a better understanding of the morphopathological mechanism involved in vein graft failure.
author	Velazquez-Gauna,Sergio E Soto-Domínguez,Adolfo Quiroga-Garza,Alejandro Reyes-Hernández,Cynthia G Chavez-Reyes,Arturo Morales-Avalos,Rodolfo Montes-de-Oca-Luna,Roberto Guzmán-López,Santos Elizondo-Omaña,Rodrigo E
author_facet	Velazquez-Gauna,Sergio E Soto-Domínguez,Adolfo Quiroga-Garza,Alejandro Reyes-Hernández,Cynthia G Chavez-Reyes,Arturo Morales-Avalos,Rodolfo Montes-de-Oca-Luna,Roberto Guzmán-López,Santos Elizondo-Omaña,Rodrigo E
author_sort	Velazquez-Gauna,Sergio E
title	Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
title_short	Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
title_full	Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
title_fullStr	Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
title_full_unstemmed	Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat
title_sort	histomorphometric and immunohistochemical study of early adaptative response of the vascular wall in a termino-terminal microsurgical model of femoral vessels in wistar rat
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200016
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Histomorphometric and Immunohistochemical Study of Early Adaptative Response of the Vascular Wall in a Termino-Terminal Microsurgical Model of Femoral Vessels in Wistar Rat

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